| Clone | BA3R |
| Isotype | IgG2b |
| Product Type | Monoclonal Antibody |
| Units | 100 µg |
| Host | Mouse |
| Species reactivity | ChickenHumanMouseRabbitRat |
| Application | Dot blotELISAWestern Blotting |
BackgroundActins are highly conserved proteins that are involved in cell motility, structure and integrity, processes that are crucial for tissue development and the development of organism. The actin cytoskeleton is one of the principal drivers of cell motility and is capable of responding to complex signaling cascades. Recent evidence suggests that it may play key roles in regulating apoptosis and aging. Beta actin is one of six different actin isoforms which have been identified. Like GAPDH,-actin is constitutively expressed at high levels in almost all tissues and cell lines making it ideal for use as a loading control marker in immunoblots.
Source
Immunogen: -Actin N-terminal peptide-KLH conjugate
Product
Formulation: 10mM PBS, pH 7.2, with 0.05% Sodium Azide
Purification Method: Protein A/G Chromatography
Concentration: See vial for concentration
ApplicationsWB (with ECL): 1:1000-10,000 dilutions (incubate for 1 hour at room temperature)IS: 1:500-2000 dilutionOptimal concentration should be evaluated by serial dilutions.
Functional Analysis: Western Blotting
Positive Control: Recognizes native and denatured forms of β-Actin (~42kDa)
StorageProduct should be stored at -20ºC. Aliquot to avoid freeze/thaw cycles
Product Stability: Products are stable for one year from purchase when stored properly
Shipping Conditions: Ship at ambient temperature, freeze upon arrival
CautionThis product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
References1.Ponte P., Ng S.Y., Engel J., Gunning P., Kedes L., Evolutionary conservation in the untranslated regions of actin mRNAs: DNA sequence of a human beta-actin cDNA.Nucleic Acids Res. 12:1687-1696(1984)2.Nakajima-Iijima S., Hamada H., Reddy P., Kakunaga T.,Molecular structure of the human cytoplasmic beta-actin gene: interspecies homology of sequences in the introns.Proc. Natl. Acad. Sci. U.S.A. 82:6133-6137(1985)3.Procaccio V., Salazar G., Ono S., Styers M.L., Gearing M., Davila A., Jimenez R., Juncos J., Gutekunst C.-A., Meroni G., Fontanella B., Sontag E., Sontag J.-M., Faundez V., Wainer B.H. A mutation of beta -actin that alters depolymerization dynamics is associated with autosomal dominant developmental malformations, deafness, and dystonia.Am. J. Hum. Genet. 78:947-960(2006) [PubMed: 16685646] [Abstract]
Protein Reference(s)
Database Name: SwissProt
Accession number: P60709
Species Accession: Human
Safety Datasheet(s) for this product:NM_Sodium Azide/wp-content/uploads/SDS/Antibody SDS with Sodium Azide Noridic-MUbio.pdf
