| ProductType | Three-ColorReagents(FITC/RPE/Cy5RPE) |
| Units | 50Tests |
| Host | Mouse |
| Speciesreactivity | Human |
| Application | FlowCytometryImmunofluorescence |
BackgroundTheCD3epitopeisexpressedontheepsilonchainoftheCD3/Tcellantigenreceptor(TcR)complex.CD3ispresenton65-85%ofthymocytesandhasamitogeniceffectonperipheralbloodTcells.CD3indentifieshumanTcellsexpressingthe22-28,000M.W.surfaceantigen.CD4identifieshumanhelper/inducerTcellsexpressingthe60,000M.W.surfaceantigen(HLAclassIIreactive).CD4ispresentinlowdensityonmonocytes.IdentificationofCD8onhumancytotoxic/suppressorTcellsexpressingthe32and43,000M.W.surfaceantigens.
Synonyms:CD3/4/8
Source
Immunogen:CD4=DerivedfromthehybridizationofmouseNS-1myelomacellswithspleencellsfromBALB/cmiceimmunizedwithhumanperherialbloodTlymphocytes.CD3=DerivedfromthehybridizationofmouseNS-1myelomacelsswithspleencellsfromBALB/cmiceimmunizedwithhumanthymocytes.CD8=DerivedfromthehybridizationofmouseNS-1myelomacellswithspleencellsfromBALB/cmiceimmunizedwithhumanperherialbloodTlymphocytes.
Product
ProductForm:RPE-Cy-5
Formulation:Providedassterilefilteredsolutioninphosphatebufferedsalinewith0.08%sodiumazideand0.2%carrierprotein
PurificationMethod:AffinityChromatography
Concentration:Titeredforflowcytometry
ApplicationsPBMC:Add15µlof3-ColorTMantibodyreagent/10^6PBMCin100µlPBS.Mixgentlyandincubatefor15minutesat2°to8°C.WashtwicewithPBS.WashtwicewithPBSorfixwith0.5%v/vofparaformaldehydeinPBSandanalyze.WHOLEBLOOD:Add15µlofExalphaBIOLOGical"s3-ColorTMantibodyreagentMAB/100µlofwholeblood.Mixgentlyandincubatefor15minutesatroomtemperature20°C.Lysethewholeblood.WashtwicewithPBSandanalyzeorfixwith0.5%v/vofparaformaldehydeinPBSandanalyze.Seeinstrumentmanufacturer’sinstructionsforLysedWholeBloodandImmunofluorescenceanalysiswithaflowcytometerormicroscope.
FunctionalAnalysis:FlowCytometryStaining
StorageProductshouldbestoredat4-8ºC.DONOTFREEZE
ProductStABIlity:Reagentsarestablefortheperiodshownontheviallabelwhenstoredproperly
ShippingConditions:RoomTemperature
CautionThisproductisintendedFORRESEARCHUSEONLY,andFORTESTSINVITRO,notforuseindiagnosticortherapeuticproceduresinvolvinghumansoranimals.Itmaycontainhazardousingredients. PleaserefertotheSafetyDataSheets(SDS)foradditionalinformationandproperhandlingprocedures.Disposeproductremaindersaccordingtolocalregulations.Thisdatasheetisasaccurateasreasonablyachievable,butNordic-Mubioacceptsnoliabilityforanyinaccuraciesoromissionsinthisinformation.
References1.OrfaoA,GonzálezdeBuitragoJMLacitometríadeflujoenellaboratorioclínico.SociedadespañoladebioquímicaclínicaypatologíaMolecular1995.2.Stetler-StevensonM.Flowcytometryanalysisoflymphomasandlymphoproliferativedisorders.SeminHematol2001Apr;38(2):111-23.3.MenéndezP,etal.Comparisonbetweenalyse-and-then-washmethodandalyse-non-washtechniquefortheenumerationofCD34+hematopoieticProgenitorcells.Cytometry(Comm.Clin.Cytometry)34:264-271(1998)4.GratamaJW,MenéndezP,KraanJ,OrfaoA.LossofCD34+hematopoieticprogenitorcellsduetowashingcanbereducedbytheuseoffixative-freeerytrocytelysingreagents.JImmunol.Methods239:13-23(2000)5.ProtectionofLaboratoryWorkersfromoccupationallyacquiredinfections.Secondedition;approvedguideline(2001).VillanovaPA:NationalCommitteeforClinicalLaboratoryStandards;DocumentM29-A2.6.Proceduresforthecollectionofdiagnosticbloodspecimensbyvenipuncture-approvedstandard;Fifthedition(2003).WaynePA:NationalCommitteeforClinicalLaboratoryStandards;DocumentH3-A5.7.Clinicalapplicationsofflowcytometry:Qualityassuranceandimmunophenotypingoflymphocytes;approvedguideline(1998).WaynePA:NationalCommitteeforClinicalLaboratoryStandards;DocumentH42-A.8.LimaMetal.TCRαβ+/CD4+LargeGranularLymphocytosis.AnewClonalT-CellLymphoprolipherativeDisorder.AmericanJournalofPathology,163(2):763-771(2003)9.GorczyzaW.etal.AnapproachtodiagnosisofT-celllymphoprolipherativedisordersbyflowcytometry.Cytometry(Clinicalcytometry)50:177-190(2002)10.BraylanRC,OrfaoA,BorowitzMJ,DavisBH.Optimalnumberofreagentsrequiredtoevaluatehematolymphoidneoplasias:resultsofaninternationalconsensusmeeting.Cytometry46:23-7(2001)11.JenningsCD,FoonKA.Recentadvancesinflowcytometry:applicationtothediagnosisofhematologicmalignancy.Blood90(8):2863-2892(1997)12.Reichertetal.LymphocytesubsetreferencerangesinadultCaucasians.ClinImmunolImmunopathol60:190-208(1991)13.PrinceHKetal.InfluenceofracialbackgroundonthedistributionofT-cellsubsetsandLeu-11positivelymphocytesinhealthyblooddonors.DiagnImmunol.3:33-39(1985)14.KotyloPKetal.Referencerangesforlymphocytesubsetsinpediatricpatients.AmJClinPathol100:111-5(1993)Cy-5PortionsofthisproductismanufacturedunderlicensefromCarnegieMellonUniversity,U.S.PatentNumber5,268,486.
ProteinReference(s)
DatabaseName:UniProt
Accessionnumber:P07766,P01730,P01732
SpeciesAccession:Human
SafetyDatasheet(s)forthisproduct:NM_SodiumAzide/wp-content/uploads/SDS/AntibodySDSwithSodiumAzideNoridic-MUbio.pdf
Nordic的纯化的牛IgG可以在各种免疫测定（包括免疫扩散，免疫电泳，ELISA，Western印迹，斑点免疫结合测定（DIBA），血细胞凝集和细胞结合测定）中用作参考抗原，校准物，包被蛋白和封闭剂。该产品仅用于研究目的，并且用于体外测试，不适用于涉及人或动物的诊断或治疗程序。它可能包含有害成分。请参阅安全数据表（SDS）了解更多信息和正确的处理程序。根据当地法规处理产品残留物。此数据表的准确性尽可能合理，但Nordic-MUbio对本信息中的任何准确性或遗漏概不负责。
