CD15, humanCatalogue number: CD15-1

Clone	MMA/LeuM1
Isotype	IgM
Product Type	Primary Antibodies
Units	1 ml
Host	Mouse
Application	Flow CytometryImmunohistochemistry (paraffin)

BackgroundThe antibody against CD15 CD15 is a helpful tool for the identification of Reed-Sternberg-Cells in Hodgkin's disease. Occasionally CD15is exprimed also in large cell B- and T-cell lymphoma. CD15 or X-Hapten plays a role in mediating phagocytosis, bacterial activity and chemotaxis. It is present on >95% of granulocytes including neutrophils and eosinophils and to a lesser degree monocytes. It is also present on Reed-Sternberg cells and on some epithelia. Human CD15 (3-fucosyl-N-acetyllactosamine).

Source

Immunogen: U937 histiocytic cell line

ProductAntibody solution in stabilizing phosphate buffer pH 7.3. Contains 0.09 % sodium azide**. The volume is sufficient for at least 100 immunohistochemical tests (100 µl working solution / test). Use appropriate antibody diluent e.g. BIOLOGOArt .No. PU002.

Purification Method: Antibody solution in stabilizing phosphate buffer pH 7.3. Contains 0.09 % sodium azide**. The volume is sufficient for at least 100 immunohistochemical tests (100 µl working solution / test). Use appropriate antibody diluent e.g. BIOLOGOArt .No. PU002.

Concentration: 50 µg/ml

Secondary Reagents: As secondary reagents we recommend the use of biotinylated anti-mouse IgM antibody (Art. No. BA-2020) in combination with streptavidin conjugates or ABC systems.

Specificity

Species Reactivity: Human

ApplicationsIHC(P), FACS

Incubation Time: 60 min at RT

Working Concentration: (liquid conc.) 1:15-1:25

Pre-Treatment: Formaldehyde-fixed material should be treated with Unmasking Solution C, citrate buffered solutionpH 6 (Art. No. DE000) at 96-100°C followed by short term treatment with Pepsin (0.1%)

Positive Control: Reed-Sternberg cells in Hodgkin's disease

Storage2-8°C

CautionThis product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but Nordic-MUbio​ accepts no liability for any inaccuracies or omissions in this information.

References1. Skubitz K. et al. In: White Cell Differentiation Antigens. New York / Oxford University Press 1989; 800-805.

Safety Datasheet(s) for this product:NM_Sodium Azide/wp-content/uploads/SDS/Antibody SDS with Sodium Azide Noridic-MUbio.pdf

Nordic的纯化的牛IgG可以在各种免疫测定（包括免疫扩散，免疫电泳，ELISA，Western印迹，斑点免疫结合测定（DIBA），血细胞凝集和细胞结合测定）中用作参考抗原，校准物，包被蛋白和封闭剂。该产品仅用于研究目的，并且用于体外测试，不适用于涉及人或动物的诊断或治疗程序。它可能包含有害成分。请参阅安全数据表（SDS）了解更多信息和正确的处理程序。根据当地法规处理产品残留物。此数据表的准确性尽可能合理，但Nordic-MUbio对本信息中的任何准确性或遗漏概不负责。