Mouse anti Green Fluorescent Protein (GFP)Catalogue number: X2678M

Clone	GF28R
Isotype	IgG1
Product Type	Monoclonal Antibody
Units	100 µg
Host	Mouse
Application	Dot blotELISAImmunohistochemistryImmunoprecipitationWestern Blotting

BackgroundGFP protein is an energy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. Fluoresces in vivo upon receiving energy from the Ca2+-activated photoprotein aequorin. Green fluorescent protein has been engineered to produce a vast number of variously colored mutants, fusion proteins, and biosensors. Fluorescent proteins and its mutated allelic forms, blue, cyan and yellow have become a useful and ubiquitous tool for making chimeric proteins, where they function as a fluorescent protein tag. Typically they tolerate N- and C-terminal fusion to a broad variety of proteins. They have been expressed in most known cell types and are used as a noninvasive fluorescent marker in living cells and organisms. They enable a wide range of applications where they have functioned as a cell lineage tracer, reporter of gene expression, or as a measure of protein-protein interactions. Reacts with Native and Dentured froms of GFP and its varients such as EGFP, YFP, EYFP, CFP.

Source

Immunogen: GFP from the jellyfish Aequorea Victoria N-terminal peptide-KLH conjugates

Product

Product Form: Unconjugated

Formulation: 10mM PBS, pH 7.2, with 0.05% Sodium Azide

Purification Method: Protein A/G Chromatography

Concentration: See vial for concentration

ApplicationsWB (with ECL): 1:1000-3000 dilution (incubate for 1 hour at room temperature)IS: 1:500-2000 dilutionOptimal concentration should be evaluated by serial dilutions.

Functional Analysis: Western Blotting

Positive Control: Recognizes native and denatured forms of GFP and its variants such as: EGFP, YFP, EYFP, and CFP

StorageProduct should be stored at -20ºC. Aliquot to avoid freeze/thaw cycles

Product Stability: Products are stable for one year from purchase when stored properly

Shipping Conditions: Ship at ambient temperature, freeze upon arrival

CautionThis product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but Nordic-MUbio​ accepts no liability for any inaccuracies or omissions in this information.

Safety Datasheet(s) for this product:NM_Sodium Azide/wp-content/uploads/SDS/Antibody SDS with Sodium Azide Noridic-MUbio.pdf

Nordic的纯化的牛IgG可以在各种免疫测定（包括免疫扩散，免疫电泳，ELISA，Western印迹，斑点免疫结合测定（DIBA），血细胞凝集和细胞结合测定）中用作参考抗原，校准物，包被蛋白和封闭剂。该产品仅用于研究目的，并且用于体外测试，不适用于涉及人或动物的诊断或治疗程序。它可能包含有害成分。请参阅安全数据表（SDS）了解更多信息和正确的处理程序。根据当地法规处理产品残留物。此数据表的准确性尽可能合理，但Nordic-MUbio对本信息中的任何准确性或遗漏概不负责。