6.5-270 kDa Protein Marker contains 10 prestained proteins ranging from 6.5 to 270 kDa. The 30 kDa and 270 kDa proteins are covalently coupled to an orange dye and the 52 kDa protein is covalently coupled to a green dye. The other 7 proteins are covalently coupled to a blue dye. Coloured protein bands can be seen in SDS-PAGE after transferring to a PDVF, nylon or nitrocellulose membrane.

Target	6.5-270 kDa Protein Marker
Tested Applications	SDS-PAGE, WB
Storage	Store at 4 °C for up to 3 months. For long-term storage, store at -20 °C. Avoid repeated freeze/thaw cycles.
Buffer	62.5 mM Tris-H3PO4 (pH 7.5), 1 mM EDTA, 2% SDS, 10 mM DTT, 1 mM NaN3, 33% glycerol.
Directions for use	3 µl/well for clear visualisation during electrophoresis on a 15-well mini-gel5 µl/well for clear visualisation during electrophoresis on a 10-well mini-gel2-3 µl/well for general Western transferring Increase the volume for thicker (> 1.5 mm) or larger gels. Do not heat, dilute or add reducing agents before loading.
Availability	Shipped within 5-10 working days.
Note	This product is for research use only.

Research Articles on 6.5-270 kDa Protein Marker 26S Proteasome Complex Subunit SEM1 (SEM1) shRNA abx972681  Specificity: SHFM1 shRNA Plasmid (Mouse) contains 3 different target-specific plasmids each encoding 19-23 nt (plus hairpin) shRNAs designed to knock down gene expression. Each plasmid contains a resistance gene for the selection of cells stably expressing shRNA. Form: Lyophilized