ADAAssayKitisanAssayKitbyEnzymaticMethod.Basedonthebottletype,productmodelcanbeclassifiedintoHitachi7170,Hitachi7060,Hitachi7020,Beckman,Toshiba,Mindray,DuPont,Innova,Siemens,KHB,Abbott,Universaltypes,etc.ThisreagentisintendedfortheinvitroquantitativedeterminationofADAinhumanserum.Mainlyusedformonitoringanddiagnosisofliverdiseases.DeaminationofadenosinebytheenzymeADA(ADA)producesinosine,thencoupledbytheactionofpurinenucleosidephosphorylase(PNP)togeneratehypoxanthine.UricAcidandhydrogenperoxideformedfromhypoxanthineunderOxidationofxanthineoxidase(XOD).Finally,peroxidase(POD)catalyzesthereactionamongH2O2,4-aminoantipyrine(4-AAP)andN-Ethyl-N-(2-hydroxy-3-sulfopropyl)-3-methylaniline(EHSPT)toformFuchsiacoloredquinones.TheADAactivitycanbecalculatedbymeasuringtheincreaserateofcoloredquinonesabsorbanceat550nmwavelength.

R1:DetectionReagent1	R2:DetectionReagent2
TrisbufferPH7.5→50mmol/L	TrisbufferPH7.5→50mmol/L
4-AAP→>3.0mmol/L	Adenosine→20mmol/L
POD→0.7KU/L	Sodium3-(N-ethyl-3-methylanilino)
XOD→0.3KU/L	-2-hydroxypropanesulfonate→2.0mmol/L
PNP→0.15KU/L

26S Proteasome Complex Subunit SEM1 (SEM1) shRNA abx972681  Specificity: SHFM1 shRNA Plasmid (Mouse) contains 3 different target-specific plasmids each encoding 19-23 nt (plus hairpin) shRNAs designed to knock down gene expression. Each plasmid contains a resistance gene for the selection of cells stably expressing shRNA. Form: Lyophilized