3.5-245 kDa Protein Marker contains 13 prestained proteins ranging from 3.5 to 245 kDa. The 25 kDa protein is covalently coupled to a green dye and the 75 kDa protein is covalently coupled to a red dye. The other 11 proteins are covalently coupled to a blue dye. Coloured protein bands can be seen in SDS-PAGE after transferring to a PDVF, nylon or nitrocellulose membrane.

Target	3.5-245 kDa Protein Marker
Tested Applications	SDS-PAGE, WB
Storage	Store at 4 °C for up to 3 months. For long-term storage, store at -20 °C. Avoid repeated freeze/thaw cycles.
Buffer	20 mM Tris-phosphate, pH 7.5, 2% SDS, 0.2 mM DTT, 3.6 M Urea, and 15% Glycerol.
Directions for use	3 µl/well for clear visualisation during electrophoresis on a 15-well mini-gel5 µl/well for clear visualisation during electrophoresis on a 10-well mini-gel2-3 µl/well for general Western transferring Increase the volume for thicker (> 1.5 mm) or larger gels. Do not heat, dilute or add reducing agents before loading.
Availability	Shipped within 5-10 working days.
Note	This product is for research use only.

Research Articles on 3.5-245 kDa Protein Marker 26S Proteasome Complex Subunit SEM1 (SEM1) shRNA abx972681  Specificity: SHFM1 shRNA Plasmid (Mouse) contains 3 different target-specific plasmids each encoding 19-23 nt (plus hairpin) shRNAs designed to knock down gene expression. Each plasmid contains a resistance gene for the selection of cells stably expressing shRNA. Form: Lyophilized