This liquid substrate for peroxidase consists of tetramethylbenzidine (TMB) plus dilute hydrogen peroxide in an aqueous single-reagent stabilized form.The reagent has been specifically formulated for measuring peroxidase on membranes and other surfaces.When reacted with peroxidase, the result is an insoluble blue reaction product.This reagent is not designed for use in microwell applications. PolyTek，抗兔聚合HRP已开发，具有成本效益，可提供最清洁，最一致的染色。该系统基于聚合的过氧化物酶标记，该标记从等式中消除了生物素，从而消除了背景染色的主要原因。