| ProductType | ControlLysate |
| Units | 100µl |
| Application | WesternBlotWesternBlotting |
BackgroundTotalcellIysatesareusefulasbothpositiveandnegativecontrolsinimmunoblotting.A431,ahumanepidermoidcarcinomacellline,andtheEGF-stimulatedA431Iysatesareusedasnegativeandpositivecontrols,respectively,whenstudyingthephosphorylationcascadeinitiatedbyligandbindingtoreceptortyrosinekinases.BothA431andnormalhumanfibroblastsarevaluablepositivecontrolsforantibodiestoproteinsexpressedinthesecells.Themousefibroblastcellline3T3anditsRSV-transformedcounterpartserveasnegativeandpositivecontrols,respectively,forantibodiestoproteinsthatarephosphorylatedasaresultoftransformationbyRSV(Roussarcomavirus).MADIn-Darbybovinekidney(MDBK)cellIysatedisplaysproteinsexpressedinbovinekidneyandPC12(ratphaeochromocytoma)cellIysatecanbeexpectedtomirrortheproteinexpressionofcellsandtissuesofneuroectodermalorigin.Likewise,JurkatcellsexpresstheproteinsuniquetohumanT-cells.Ratbrainisawidelyusedpositivecontrolforthoseproteinsthatareexclusivelyexpressedinbrain.AtrightisanexampleoftheuseofpositiveandnegativecontrolIysatesinwesternblotting.Theblotof3T3cellIysate(lane1)andRSV-transformed3T3(lane2)wasprobedwithanti-phosphotyrosineperoxidaseconjugatetorevealthoseproteinsthataretyrosine-phosphorylatedinresponsetotransformationby
Synonyms:NormalHumanFibroblastcelllysate
Product
ProductForm:Unconjugated
Formulation:ProvidedassolutioninLaemmlielectrophoresissamplebuffer.Samplesneedtobeboiledfor3-5minutes,beforeloadingthegel.
Concentration:Seevialforconcentration
ApplicationsSuggesteduse:Avolumeof10-25ulissuggestedforimmunobloting.
FunctionalAnalysis:WesternBlotting
StorageProductshouldbestoredat-20ºC.Aliquottoavoidfreeze/thawcycles
ProductStABIlity:Reagentsarestablefortheperiodshownontheviallabelwhenstoredproperly
ShippingConditions:RoomTemperature
CautionThisproductisintendedFORRESEARCHUSEONLY,andFORTESTSINVITRO,notforuseindiagnosticortherapeuticproceduresinvolvinghumansoranimals.Itmaycontainhazardousingredients. PleaserefertotheSafetyDataSheets(SDS)foradditionalinformationandproperhandlingprocedures.Disposeproductremaindersaccordingtolocalregulations.Thisdatasheetisasaccurateasreasonablyachievable,butExalphaBIOLOGicalsacceptsnoliabilityforanyinaccuraciesoromissionsinthisinformation.
ProteinReference(s)
DatabaseName:UniProt
Accessionnumber:O00622
SpeciesAccession:Human
Exalpha血清是凝结后残留的全血液体部分。通过离心将该液体部分与凝结的细胞和凝血因子分离。它包含蛋白质的混合物，包括抗体（免疫球蛋白），血清白蛋白，α和β球蛋白以及调节蛋白，例如激素和酶。我们从人类，灵长类和兽医物种中提取的血清和牛奶可用于多种用途；研究血清或牛奶的正常蛋白质组成，作为参考蛋白质，阻断剂或阴性对照。
