Background
The reactivity of the antiserum is directed to the Fc and Fab subunits of the major Immunoglobulin isotypes. It does not react with any non-Ig protein in monkey serum, as tested by immunoelectrophoresis and double radial immunodiffusion. In enzyme-immunocytochemical and immunohistochemical staining for the detection of IgG, antigen or antibody, of appropriately treated cell and tissue substrates at the cellular and subcellular level. In non-isotopic assay methodology (e.g. ELISA) to identify and measure a specific IgG in monkey serum or other body fluid. In electron microscopy, since the complex between the conjugated antibody and the antigen also has electron-dense properties. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Working dilutions for histochemical and cytochemical use are usually between 1:100 and 1: 500; in ELISA and comparable non-precipitating antibody-binding assays between 1:2,000 and 1:8,000.
Species Reactivity
Monkey
Product Usage
Horseradish peroxidase conjugated IgG fraction of polyclonal Monkey antiSerum to Rabbit immunoglobulins IgG, IgA and IgM, heavy and light chains
Product Type
Secondary Antibodies
Applications
ELISA,Immunocytochemistry,Immunohistochemistry (paraffin),Dot blot,Immunoblotting
Fields of Interest
Veterinary
Product Source
Purified immunoglobulin IgG, IgA and IgM fractions isolated from pooled rhesus monkey serum. Freund
Product Formulation
Peroxidase-coupled purified hyperimmune rabbit IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2). No preservative added, as it may interfere with the antibody activity
Product Specificity
Inter-species cross-reactivity is a normal feature of antibodies to immunoglobulins, since Ig of different species frequently share antigenic determinants. Cross-reactivity of this antiSerum has not been tested in detail.
Storage Conditions
The lyophilized conjugate is shipped at ambient temperature and may be stored at +4
Exalpha血清是凝结后残留的全血液体部分。通过离心将该液体部分与凝结的细胞和凝血因子分离。它包含蛋白质的混合物,包括抗体(免疫球蛋白),血清白蛋白,α和β球蛋白以及调节蛋白,例如激素和酶。我们从人类,灵长类和兽医物种中提取的血清和牛奶可用于多种用途;研究血清或牛奶的正常蛋白质组成,作为参考蛋白质,阻断剂或阴性对照。