OVERVIEW:

Synonyms	MonoclonalCD274antibody,Anti-CD274antibody,PDCD1L1antibody,PDCD1LG1antibody,PDL1antibody,CD274antibody,CD-274antibody,CD274,CD-274,CD274,B7-Hantibody,B7H1antibody,PD-L1antibody
Applications	FC,IHC-F,IP
Immunogen	CD274antibodywasraisedinRatusingMouseCD274transfectedL5178Ycellsastheimmunogen

SPECIFICATIONS:

Host	Rat
Clone	MIH6
Isotype	IgG2a
MethodofPurification	CD274antibodywaspurifiedbyProteinGaffinitychromatography
Form&Buffer	SuppliedinPBSbufferwith0.09%SodiumAzide(NaN3)addedasapreservative
Concentration	1.0mg/ml

STORAGE&SAFETY:

Storage	Storeat4degCforshorttermstorageoraliquotandfreezeat-20degCforlongtermstorage

UsageAssayInformation:

UsageRecommendations	FC,IHC-F,IP

GENERALINFORMATION:

ProductUse	MonoclonalCD274antibodyspecificforHumanCD274foruseinFlowCytometry,ImmunohistochemistryandImmunoprecipitation.
BIOLOGicalSignificance	CD274isatransmembraneproteinthathasbeenspeculatedtoplayamajorroleinsuppressingtheimmunesystemduringparticulareventssuchaspregnancy,tissueallografts,autoimmunediseaseandotherdiseasestatessuchashepatitis.Normallytheimmunesystemreactstoforeignantigenswherethereissomeaccumulationinthelymphnodesorspleenwhichtriggersaproliferationofantigen-specificCD8+Tcell.TheformationofCD274receptor/PD-L1orB7.1receptor/PD-L1ligandcomplextransmitsaninhibitorysignalwhichreducestheproliferationoftheseCD8+TcellsatthelymphnodesandsupplementarytothatCD274isalsoabletocontroltheaccumulationofforeignantigenspecificTcellsinthelymphnodesthroughapoptosiswhichisfurthermediatedbyalowerregulationofthegeneBcl-2.
Expression	CD274ishighlyexpressedintheheart,skeletalmuscle,placentaandlung.Weaklyexpressedinthethymus,spleen,kidneyandliver.ExpressedonactivatedT-andB-cells,dendriticcells,keratinocytesandmonocytes.CD274isaSingle-passtypeImembraneproteinandamemberoftheBTN/MOGgroupintheimmunoglobulinsuperfamily.
ImplicationsinDisease	UpregulationofCD274mayallowcancerstoevadethehostimmunesystem.Ananalysisof196tumorspecimensfrompatientswithrenalcellcarcinomafoundthathightumorexpressionofCD274wasassociatedwithincreasedtumoraggressivenessanda4.5-foldincreasedriskofdeath.OvariancancerpatientswithhigherexpressionofCD274hadasignificantlypoorerprognosisthanthosewithlowerexpression.CD274expressioncorrelatedinverselywithintraepithelialCD8+T-lymphocytecount,suggestingthatCD274ontumorcellsmaysuppressantitumorCD8+Tcells.ThishasencourageddevelopmentofCD274inhibitorswhichAsofApril2013havestartedclinicaltrials.Theeffectmightbetumortypedependant;astudyonpatientswithnon-smallcelllungcancershowedthatgreaterPD-L1proteinandmRNAexpressionisassociatedwithincreasedlocallymphocyticinfiltrateandlongersurvival.
SequenceInformation	CD274contains290aminoacidsandhasamolecularweightof33275Da.Itcontains1Ig-likeC2-type(immunoglobulin-like)domain.
Post-TranslationalModifications	CD274undergoesglycosylationatAsn35,Asn192,Asn200andAsn219.
DatabaseLinks	EntrezGene:29126Human,Omim:605402Human,SwissProt:Q9NZQ7Human,Unigene:521989Human

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