Type

Recombinant

Description

IL-15 isanimmunomodulatingcytokinethatstimulatestheproliferationofTlymphocytesandsharesmanyBIOLOGicalpropertieswithIL-2. IL-15 exertsitsbiologicalactivitiesprimarilyonTcells.Itisalsoessentialinthedevelopment,survivalandactivationofNKcells.IncreasedexpressionofIL-15 hasbeenlinkedtorheumatoidarthritis,inflammatoryboweldisease,anddiseasesaffiliatedwithretrovirusesHIVandHTLV-I.HumanIL-15 isbiologicallyactiveonmousecellsasmeasuredbythedose-dependentstimulationoftheproliferationofmouseCTLLcells.RecombinantMurineIL-15 isa13.3 kDaproteinconsistingof115 aminoacidresidues.

AminoAcidSequence

MNWIDVRYDLEKIESLIQSIHIDTTLYTDSDFHPSCKVTAMNCFLLELQVILHEYSNMTLNETVRNVLYLANSTLSSNKNVAESGCKECEELEEKTFTEFLQSFIRIVQMFINTS

Source

E.coli

Purity

98%

BiologicalActivity

TheED₅₀wasdeterminedbythestimulationoftheproliferationofmurineCTLL-2 cellsis<5.0 ng/ml,correspondingtoaspecificactivityof>2×10⁵units/mg.

Endotoxin

Endotoxinlevelis<0.1 ng/μgofprotein(<1EU/μg).

Reconstitution

Centrifugethevialpriortoopening.Reconstituteinwatertoaconcentrationof0.5–1.0 mg/ml.Donotvortex.Thissolutioncanbestoredat2–8°Cforupto1 week.Forextendedstorage,itisrecommendedtofurtherdiluteinabuffercontainingacarrierprotein(example0.1%BSA)andstoreinworkingaliquotsat –20°Cto –80°C.

Storage/Expiration

 –20°C