Type
Recombinant
Description
IFN-ωisatypeI interferonthatcanbeinducedbyvirus-infectedleukocytes.MembersofthetypeI interferonfamily,whichincludesIFN-α,IFN-β,andIFN-ω,signalthroughtheIFNAR-1/IFNAR-2 receptorcomplex,andexertantiviralandantiproliferativeactivities.IFN-ωexhibitsabout75%sequencehomologywithIFN-α,andcontainstwoconserveddisulfidebondsthatarenecessaryforfullBIOLOGicalactivity.RecombinantHumanIFN-ωisa19.9 kDaproteinconsistingof172 aminoacidresidues.
AminoAcidSequence
CDLPQNHGLLSRNTLVLLHQMRRISPFLCLKDRRDFRFPQEMVKGSQLQKAHVMSVLHEMLQQIFSLFHTERSSAAWNMTLLDQLHTGLHQQLQHLETCLLQVVGEGESAGAISSPALTLRRYFQGIRVYLKEKKYSDCAWEVVRMEIMKSLFLSTNMQERLRSKDRDLGSS
Source
E.coli
Purity
98%
BiologicalActivity
TheED50wasdeterminedbyacytotoxicityassayusingHumanTF-1 cellsis0.01 ng/ml,correspondingtoaspecificactivityof1×108units/mg.
Endotoxin
Endotoxinlevelis<0.1 ng/μgofprotein(<1EU/μg).
Reconstitution
Centrifugethevialpriortoopening.Reconstituteinwatertoaconcentrationof1.0 mg/ml.Donotvortex.Thissolutioncanbestoredat2–8°Cforupto1 week.Forextendedstorage,itisrecommendedtofurtherdiluteinabuffercontainingacarrierprotein(example0.1%BSA)andstoreinworkingaliquotsat –20°Cto –80°C.
Storage/Expiration
–20°C
