Type

Recombinant

Description

Total76 AA.MW:8,7 kDa(calculated).UniProtKBacc.no.P13500(24–99)ProteinidentityconfirmedbyLC-MS/MS.

AminoAcidSequence

QPDAINAPVTCCYNFTNRKISVQRLASYRRITSSKCPKEAVIFKTIVAKEICADPKQKWVQDSIDHLDKQTQTPKT

Source

E.coli

Purity

PurityasdeterminedbySDSPAGE:>97%

BIOLOGicalActivity

EC50=0.067nMdeterminedbyMigrationofrecombinantCCR2containing cells

Endotoxin

<0.01 EU/1µg

Reconstitution

Addsterilewatertoprepareaworkingstocksolutionofapproximately0.1 mg/mlandletthelyophilizedpelletdissolvecompletely.

Applications

Westernblotting,ELISA,Cellcultureand/oranimalstudies,Biologicallyactiveprotein

Shipping

Atambienttemperature.Uponreceipt,storetheproductatthetemperaturerecommended below.

Storage/Expiration

Storethelyophilizedproteinat –20to –70°C.Lyophilizedproteinremainsstableuntiltheexpirydatewhenstoredat-20 to –70°C.Aliquotreconstitutedproteintoavoidrepeatedfreezing/thawingcyclesandstoreat -20 to –70°C,theproteinisstablefor3 monthsat –20to –70°Cundersterileconditionsafterreconstitution.Reconstitutedproteincanbestoredat4 °Cfor1 monthundersterileconditionsafterreconstitution

QualityControlTest

QuantificationisdonebyA280,usingthecalculatedextinctioncoefficient(10486 M-1 cm-1).
SDS-PAGEtodeterminepurityoftheprotein.
LALTESTtodetermineendotoxin level.

Note

Thisproductisintendedforresearchuseonly.