DESCRIPTION: Oligonucleosomes purified from chicken blood cells (primarily oligomers of 3-6 units, 600-1200 bp DNA). These are core nucleosomes comprising histone octamers (two copies each of histones H3, H4, H2A, H2B), each wrapped with ~146 bp of DNA with ~50 additional bp of internucleosomal DNA. Useful for the assay of various histone methyltransferases (e.g. MLL1 Complex, MLL2 Complex, MLL4 Complex, Dot1L and NSD2) by methods employing radiolabeling with [3H]-S-adenosylmethionine (e.g. gel electrophoresis/autoradiography or filterplate/scintillation counting). Most histone methyltransferases that require nucleosomes for assay have equal or somewhat greater activity with chicken, relative to HeLa, nucleosomes.
NOTE: Each vial contains 50 µg nucleosomal DNA, determined by A260nm. At ~200 bp/nucleosome, the total weight, DNA + protein, is thus ~91 µg.
A 50ug vial of RBC nucleosomes contains over 80% more product than those from vendors that sell by total weight.
SOURCE: Chicken blood cells
SUPPLIED AS: Purified chicken nucleosomes in 20 mM HEPES pH 7.5, 1 mM EDTA, 0.5 mM PMSF, 1 mM ß-mercaptoethanol, 20% glycerol (w/v).
STORAGE: -80°C, aliquot and snap-freeze after first use.
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Substantial Discounts Available on Bulk Quantities
