ELISpot^PLUS for enumeration of cells secreting EBI3

This kit is ideal for users who want a convenient and sensitive assay. The assay is designed for the enumeration of cells secreting human EBI3. The kit includes ELISpot plates pre-coated with monoclonal antibody, biotinylated detection antibody, Streptavidin-ALP and ready-to-use BCIP/NBT-plus substrate.

The monoclonal antibodies in this kit are specific to the EBI3 subunit of IL-27 and IL-35. ELISpot assays based on antibodies to EBI3 will detect both IL-27 and IL-35.

Pre-coated plates reduce assay time and minimize variability.

Human EBI3 ELISpot^PLUS kits are also available with Streptavidin-HRP and TMB substrate. See Related products.

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Analyte Information

EBI3EBI3 is a subunit molecule that forms heterodimers with other proteins in the IL-12 family. IL-27 is a heterodimer composed of the subunits EBI3 and p28. IL-35 is a heterodimer composed of the subunits EBI3 and p35. IL-39 is a heterodimer composted of the subunits EBI3 and p19. Native IL-39 has not been detected in human.

Ordering Information

Orders to Mabtech can be placed online, by email, or by phone/fax to any of our offices.

Email:Send your order to order@mabtech.com.

Customers from USA or Canada: Please send your order to mabtech.usa@mabtech.com.

Application:	ELISpot
Reactivity:	Human
Documentation:	Datasheet/ProtocolSDS
Contents:	Biotinylated detection mAb (MT140) Streptavidin-ALP Substrate (BCIP/NBT-plus) 10 Pre-coated MSIP white plates (mAb MT23A14)
Size:	10 plates
Other formats:	Click to view all Human EBI3 products
I have compared Mabtech ELISpot assay to ELISpot assays we run at our company on regular basis "head-to-head" using identical samples. Mabtech"s kits proved to be superior to our protocol, had significantly lower background and provided better and higher detection of spots.