ThemouseapoA1ELISAPROkitprovidesallnecessaryreagentsandsolutionsrequiredtoquantifyapoA1insolution,e.g.incellculturesupernatant,andserum/plasmasamples.ThekitcontainsELISAplatespre-coatedwithmonoclonalcaptureantibodyandmatchedquantitiesofbiotinylateddetectionantibody,Streptavidin-HRP,TMBsubstrate,ELISAstandardandbuffers.
Dilutionguidelinesforserum/plasmasamplescanbefoundhere.
Pre-coatedELISAplatessavetimeandreduceassayvariABIlity.
Standardrange
0.16-10ng/ml.Thestandardrangeistherangeinwhichdeterminationsofanalyteconcentrationcanbedonewithprecision,accuracyandlinearity.
Sensitivity
Thelimitofdetectionofthisassayis0.03ng/ml.ItisthelowestconcentrationthatispossIBLetodetectbutnotnecessarilyquantifywithprecisionandaccuracy.
Dilutionrecovery
Dilutionofserum/plasmasamplesgivesamid-curverecoveryof94-107%inrepeatedexperiments.
Precision
Theintra-assayvariationis3.7%(CV).Theinter-assayvariationis4.5%(CV).
AnalyteInformation
ApoA1
ApolipoproteinA1(apoA1,alsoknownasapolipoprotein-A1,apoA1andapoA-I)isthemainproteincomponentofhighdensitylipoproteinparticles(HDL).ApoA1(28kD)ismainlyproducedintheliverandexportedintothecirculationwhereitacquireslipidsandbecomesHDL.HighlevelofapoA1isregardedasprotectiveagainstatherosclerosisandcardiovasculardisease.NormalconcentrationofapoA1inhumanplasmaisapproximately0.8-2.2g/L.
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Standardrange: | 0.16-10ng/ml |
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Size: | 2plates |
Otherformats: | ClicktoviewallMouseapoA1products |
VeryeasytouseELISAkit. Mabtech提供了多种不同格式的ELISpot试剂盒,用于检测非人类灵长类动物(NHP)样品中的细胞因子和其他免疫效应分子。
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