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Mabtech/Human Thioredoxin-1 ELISA PRO kit/3580-1HP-2/2 plates

价格
¥11700.00
货号:3580-1HP-2
浏览量:127
品牌:Mabtech
服务
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商品描述
ELISAPROkitforquantificationofThioredoxin-1insolution

ThehumanThioredoxin-1ELISAPROkitprovidesallnecessaryreagentsandsolutionsrequiredtoquantifyhumanThioredoxin-1insolution,e.g.incellculturesupernatant,andserum/plasmasamples.ThekitcontainsELISAplatespre-coatedwithmonoclonalcaptureantibody,biotinylateddetectionantibody,Streptavidin-HRP,TMBsubstrate,ELISAstandardandAssaybuffer.TheAssaybufferpreventsfalsepositiveread-outswhichmaybecausedbyheterophilicantibodiesfoundinserumandplasmasamples.

Pre-coatedELISAplatessavetimeandreduceassayvariABIlity.

Serum/Plasmasamples

ForaccuratemeasurementofTrx1bloodlevels,theuseofplasmaisrecommendedsinceserumcontainshighlevelsofTrx1releasedfromplatelets.Tominimizetheplateletcontentinplasma,anadditionalcentrifugationat10,000xgfor10minutesinconnectionwiththeplasmapreparationisrecommended.

Speciescross-reactivity

Themonoclonalantibodiesinthiskitcross-reactwithThioredoxin-1fromcow.ForquantificationofhumanThioredoxin-1,samplesshouldnotcontainbovineserum.


AnalyteInformation

Thioredoxin-1
Thioredoxin-1(Trx1)isanoxidoreductaseproteinandthusmayactasananti-oxidantbyfacilitatingthereductionofcysteineresiduesofotherproteins.Besideskeepingtheintracellularmilieuinareducedstateandbeinginvolvedinthedefenceagainstoxidativestress,thioredoxin-1specificallyregulatesseveraltranscriptionfactorsandreceptors.Increasedplasmalevelsofthioredoxin-1hasbeenassociatedwithseveralpsychiatricdisorders.

Application:
  • ELISA
Reactivity:
  • Human
Standardrange:31.25-4000pg/ml
Contents:
  • BiotinylateddetectionmAb(MT13X3)
  • Streptavidin-HRP
  • RecombinanthumanThioredoxin-1ELISAstandard
  • Substrate(TMB)
  • Buffersforwashing,dilutionandstandardreconstitution
  • 2Pre-coatedELISAstripplates(mAbMT17R6)
Size:2plates
VeryeasytouseELISAkit. Mabtech提供了多种不同格式的ELISpot试剂盒,用于检测非人类灵长类动物(NHP)样品中的细胞因子和其他免疫效应分子。
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