ThehumanThioredoxin-1ELISAPROkitprovidesallnecessaryreagentsandsolutionsrequiredtoquantifyhumanThioredoxin-1insolution,e.g.incellculturesupernatant,andserum/plasmasamples.ThekitcontainsELISAplatespre-coatedwithmonoclonalcaptureantibody,biotinylateddetectionantibody,Streptavidin-HRP,TMBsubstrate,ELISAstandardandAssaybuffer.TheAssaybufferpreventsfalsepositiveread-outswhichmaybecausedbyheterophilicantibodiesfoundinserumandplasmasamples.
Pre-coatedELISAplatessavetimeandreduceassayvariABIlity.
Serum/Plasmasamples
ForaccuratemeasurementofTrx1bloodlevels,theuseofplasmaisrecommendedsinceserumcontainshighlevelsofTrx1releasedfromplatelets.Tominimizetheplateletcontentinplasma,anadditionalcentrifugationat10,000xgfor10minutesinconnectionwiththeplasmapreparationisrecommended.
Speciescross-reactivity
Themonoclonalantibodiesinthiskitcross-reactwithThioredoxin-1fromcow.ForquantificationofhumanThioredoxin-1,samplesshouldnotcontainbovineserum.
AnalyteInformation
Thioredoxin-1
Thioredoxin-1(Trx1)isanoxidoreductaseproteinandthusmayactasananti-oxidantbyfacilitatingthereductionofcysteineresiduesofotherproteins.Besideskeepingtheintracellularmilieuinareducedstateandbeinginvolvedinthedefenceagainstoxidativestress,thioredoxin-1specificallyregulatesseveraltranscriptionfactorsandreceptors.Increasedplasmalevelsofthioredoxin-1hasbeenassociatedwithseveralpsychiatricdisorders.
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| Standardrange: | 31.25-4000pg/ml |
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| Size: | 2plates |
| Otherformats: | ClicktoviewallHumanThioredoxin-1products |
VeryeasytouseELISAkit. Mabtech提供了多种不同格式的ELISpot试剂盒,用于检测非人类灵长类动物(NHP)样品中的细胞因子和其他免疫效应分子。
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