IhavecomparedMabtechELISpotassaytoELISpotassayswerunatourcompanyonregularbasis"head-to-head"usingidenticalsamples.Mabtech"skitsprovedtobesuperiortoourprotocol,hadsignificantlylowerbackgroundandprovidedbetterandhigherdetectionofspots.
Mabtech提供了多种不同格式的ELISpot试剂盒，用于检测非人类灵长类动物（NHP）样品中的细胞因子和其他免疫效应分子。
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公司地址
苏州工业园区生物纳米园A4#216
联系电话
4000-520-616 / 18915418616
传真号码
0512-67156496
电子邮箱
info@ebiomall.com
公司网址
https://www.ebiomall.com
商品描述
BasicELISpotforenumerationofIgMsecretingBcell
MouseIgMELISpotBASICenablesenumerationofBcellssecretingtotalmouseIgMaswellasantigen-specificmouseIgM.Itcontainsmatchedquantitiesofmonoclonalcaptureanddetectionantibodies,Streptavidin-HRPandpolyclonalactivators.
Antigen-specificBcellscanbeenumeratedintwoalternativeways;bycoatingwithantigenorbyusingbiotinylatedantigeninthedetectionstep.FormemoryBcells,werecommendpre-stimulationwithR848andrecombinantmouseIL-2(bothincluded).
MouseIgMELISpotBASICisalsoavailablewithStreptavidin-ALP.SeeRelatedproducts.
Ascomplementaryproducts,weofferELISpotplatesandsubstrates.SeeRelatedproducts.
AnalyteInformation
IgM
MonomericIgM(ImmunoglobulinM)isfoundonthesurfaceofBcellswhereastheIgMmoleculessecretedfromplasmaBcellsarepentamers,i.e.fiveIgMmoleculesjoinedtogetherbytheJchain.IgMconstitutesapproximately10%ofthetotalIgconcentrationinserum.IgMisthefirstantibodyisotypeinducedinatypicalimmuneresponseandhenceistheisotypewiththelowestaffinityforantigens.Duetoitsstructure,with10antigen-bindingsitesperIgMpentamer,itcanneverthelessbinde.g.surface-boundantigenswithaveryhighAvidity.
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| Otherformats: | ClicktoviewallMouseIgMproducts |
