IhavecomparedMabtechELISpotassaytoELISpotassayswerunatourcompanyonregularbasis"head-to-head"usingidenticalsamples.Mabtech"skitsprovedtobesuperiortoourprotocol,hadsignificantlylowerbackgroundandprovidedbetterandhigherdetectionofspots.
Mabtech提供了多种不同格式的ELISpot试剂盒,用于检测非人类灵长类动物(NHP)样品中的细胞因子和其他免疫效应分子。Thiskitisidealforuserswhowantaconvenientandsensitiveassay.TheassayisdesignedfortheenumerationofcellssecretinghumanIL-17A.ThekitincludesELISpotplatespre-coatedwithmonoclonalantibody,biotinylateddetectionantibody,Streptavidin-ALPandready-to-useBCIP/NBT-plussubstrate.ApolyclonalactivatorforhumanTcells(mAbCD3-2)issuppliedasapositivecontrolforcytokinesecretion.
Pre-coatedplatesreduceassaytimeandminimizevariABIlity.
HumanIL-17AELISpotPLUSkitsarealsoavailablewithStreptavidin-HRPandTMBsubstrate.SeeRelatedproducts.
AnalyteInformation
IL-17A
Interleukin-17A(IL-17A,IL17A)isapotentproinflammatorycytokineproducedbyactivatedTh17(T-helper17)cellsandcertaincellsbelongingtotheinnateimmunesystem.Inmice,IL-17hasbeenshowntoalsobeproducedbyactivatedCD8+TcellsandγδTcells.Th17cellsplayanimportantroleinautoimmunediseasesandprotectionagainstbacteriaandfungi.IL-17Aactsonabroadrangeofcelltypestoinduceexpressionofcytokines,chemokinesandmetalloproteinases.Asaresult,secretionofIL-17Apromotesinflammatoryresponses,whichleadstotherecruitmentofneutrophils,enhancementofantibodyproductionandactivationofTcells.IncreasedexpressionofIL-17Aisseeninautoimmunediseasessuchasmultiplesclerosisandrheumatoidarthritis.Itisalsoassociatedwithasthma,psoriasis,cancerandtransplantrejection.
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Size: | 10plates |
Otherformats: | ClicktoviewallHumanIL-17Aproducts |
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