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Mabtech/Human IgA ELISpot BASIC (HRP)/3860-2H/for 4 plates

价格
¥8400.00
货号:3860-2H
浏览量:86
品牌:Mabtech
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商品描述
BasicELISpotforenumerationofcellssecretingIgA

ThehumanIgAELISpotBASICenablesenumerationofBcellssecretingtotalhumanIgAaswellasantigen-specifichumanIgA.Itcontainsamatchedpairofmonoclonalcaptureanddetectionantibodies,Streptavidin-HRPandpolyclonalactivators.

Antigen-specificBcellscanbeenumeratedintwoalternativeways;bycoatingwithantigenorbyusingbiotinylatedantigeninthedetectionstep.FormemoryBcells,werecommendpre-stimulationwithR848andrecombinanthumanIL-2(bothincluded).

HumanIgAELISpotBASICisalsoavailablewithStreptavidin-ALP.SeeRelatedproducts.

Ascomplementaryproducts,weofferELISpotplatesandsubstrates.SeeRelatedproducts.


AnalyteInformation

IgA
IgA(ImmunoglobulinA)representsonly10-15%ofthetotalIgmoleculesinserumbutisthepredominatingIgisotypeinsaliva,tears,breastmilkandinthegastrointestinalandurogenitaltract.Inserum,theIgAmoleculeiscommonlyfoundasamonomerofeitherIgA1orIgA2subclassbutcanalsoexistasdimersorlargerpolymericforms.TheIgAfoundinsecretions(secretoryIgAorsIgA)iscommonlyadimerwithtwoIgAmoleculesjoinedbytheJchain.SecretoryIgAisthemostimportantIgisotypeinmucoussurfaces,theareaofentryformostpathogens.

Application:
  • ELISpot
Reactivity:
  • Human
Contents:
  • CapturemAb(MT57)
  • BiotinylateddetectionmAb(MT20)
  • BiotinylateddetectionmAb(MT57)
  • Streptavidin-HRP
  • R848
  • RecombinanthumanIL-2
Size:for4plates

IhavecomparedMabtechELISpotassaytoELISpotassayswerunatourcompanyonregularbasis"head-to-head"usingidenticalsamples.Mabtech"skitsprovedtobesuperiortoourprotocol,hadsignificantlylowerbackgroundandprovidedbetterandhigherdetectionofspots.

Mabtech提供了多种不同格式的ELISpot试剂盒,用于检测非人类灵长类动物(NHP)样品中的细胞因子和其他免疫效应分子。
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