IhavecomparedMabtechELISpotassaytoELISpotassayswerunatourcompanyonregularbasis"head-to-head"usingidenticalsamples.Mabtech"skitsprovedtobesuperiortoourprotocol,hadsignificantlylowerbackgroundandprovidedbetterandhigherdetectionofspots.
Mabtech提供了多种不同格式的ELISpot试剂盒,用于检测非人类灵长类动物(NHP)样品中的细胞因子和其他免疫效应分子。ThehumanIgAELISpotBASICenablesenumerationofBcellssecretingtotalhumanIgAaswellasantigen-specifichumanIgA.Itcontainsamatchedpairofmonoclonalcaptureanddetectionantibodies,Streptavidin-HRPandpolyclonalactivators.
Antigen-specificBcellscanbeenumeratedintwoalternativeways;bycoatingwithantigenorbyusingbiotinylatedantigeninthedetectionstep.FormemoryBcells,werecommendpre-stimulationwithR848andrecombinanthumanIL-2(bothincluded).
HumanIgAELISpotBASICisalsoavailablewithStreptavidin-ALP.SeeRelatedproducts.
Ascomplementaryproducts,weofferELISpotplatesandsubstrates.SeeRelatedproducts.
AnalyteInformation
IgA
IgA(ImmunoglobulinA)representsonly10-15%ofthetotalIgmoleculesinserumbutisthepredominatingIgisotypeinsaliva,tears,breastmilkandinthegastrointestinalandurogenitaltract.Inserum,theIgAmoleculeiscommonlyfoundasamonomerofeitherIgA1orIgA2subclassbutcanalsoexistasdimersorlargerpolymericforms.TheIgAfoundinsecretions(secretoryIgAorsIgA)iscommonlyadimerwithtwoIgAmoleculesjoinedbytheJchain.SecretoryIgAisthemostimportantIgisotypeinmucoussurfaces,theareaofentryformostpathogens.
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Otherformats: | ClicktoviewallHumanIgAproducts |
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