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蚂蚁淘在线 / 品牌中心 / Adipogen / Adipogen/Di-ubiquitin [Ub2] (K48-linked) (human) (rec.) (TAMRA Pos1)/AG-40T-0238-C050/50 µg
商品描述
| ProductDetails | |
|---|---|
| ProductType | Protein |
| Properties | |
| Source/Host | E.coli |
| Sequence | Twohumanubiquitin(AccessionNr.P0CG47)covalentlylinkedthroughanisopeptidebondatK48residueofoneubiquitinmoleculeandtheC-terminalglycineresidueofanotherubiquitinmoleculeandconjugatedtoTAMRA. |
| Crossreactivity | Human |
| Label/Conjugates | TAMRA |
| Formulation | Liquid.In50mMHepespH7.5,150mMNaCl,2mMDTT. |
| OtherProductData | Use:Linkage-specificdi-ubiquitinisasubstrateforenzymesthatcleavetheisopeptidelinkagebetweentwoubiquitinmolecules.ThisFRET-basedDUBsubstratewithisopeptidebondsbetweenubiquitinmoietiesissuperiortogeldensitometryassays.SinceDUBsrecognizeandcleavesubstrateswithspecificstericconditions,eachsubstratevariesinthepositionofthefluorophoreandquencherandmustbeempiricallytestedforindividualenzymes.Idealforuseasasubstrateforubiquitin-specifichydrolaseswithactivitytowardK48linkages.Werecommendanassaybufferconsistingof50mMHepespH7.5,150mMNaCl,2mMDTT.Fluorescencecanbemonitoredwithanexcitationwavelengthof544nMandanemissionwavelengthof572nM.Reactionconditionswillneedtobeoptimizedforeachspecificapplication.Werecommendaninitialproteinconcentrationof100-500nM. |
| Declaration | ManufacturedbyBostonBiochem |
| ShippingandHandling | |
| Shipping | DRYICE |
| ShortTermStorage | -20°C |
| LongTermStorage | -80°C |
| HandlingAdvice | Aliquottoavoidfreeze/thawcycles. Protectfromlight. |
| Use/StABIlity | Stableforatleast1yearafterreceiptwhenstoredat-80°C. |
| Documents | |
| MSDS | No |
| ProductSpecificationSheet | |
| Datasheet |  DownloadPDF |
Withapredictedmolecularweightof17kDa,di-ubiquitiniscomposedoftwoubiquitinmonomersthatarecovalentlylinkedthroughanisopeptidebond,whichtypicallyformbetweenalysineresidueofoneubiquitinmoleculeandtheC-terminalglycineresidueofanotherubiquitinmolecule.Eachhumanubiquitinmonomeris76aminoacids(aa)inlengthandshares96%and100%aaidentitywithyeastandmouseubiquitin,respectively.Ubiquitinhassevenlysineresiduesthatcanparticipateintheformationofpoly-ubiquitinchains.Thespecificlysineresidueusedinubiquitinconjugationisthoughttodeterminethefunctionofpoly-ubiquitinationincellularprocessessuchasproteindegradation,signalingandtrafficking.
