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Protein

E. coli

Four human ubiquitin (Accession Nr. P0CG47) covalently linked through isopeptide bonds at K11 residues of one ubiquitin molecule and the C-terminal glycine residue of another ubiquitin molecule.

Human

~34kDa

≥95% (SDS-PAGE)

1mg/ml

Liquid. In sterile, deionized water.

Use: Formed with wild-type human recombinant ubiquitin and linkage-specific enzymes. Validated by Ub-AQUA, an LCMS-based technique that provides extremely accurate information on the composition of Poly-ubiquitin samples. Linkage-specific tetra-ubiquitin can be used to investigate mechanism of binding and recognition by E1 or E2 enzymes, deubiquitinating enzymes, E3 ligases or other proteins that contain ubiquitin-associated domains (UBAs) or ubiquitin-interacting motifs (UIMs). Ideal for investigating ubiquitin-binding proteins and as substrates for ubiquitin-specific isopeptidases. Reaction conditions will need to be optimized for each specific application. IMPORTANT: Heating this product in SDS-PAGE buffer or terminating reactions containing this product with heated SDS-PAGE buffer could lead to unexpected, high apparent molecular weight banding or smearing on gels that is not representative of product purity. For optimal results, we recommend incubation in SDS-PAGE buffer + DTT at

Manufactured by Boston Biochem

DRY ICE

-20°C

-80°C

Avoid freeze/thaw cycles.

Stable for at least 1 year after receipt when stored at -80°C.

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With a predicted molecular weight of 34 kDa, Tetra-ubiquitin chains are composed of four ubiquitin monomers that are covalently linked through isopeptide bonds, which typically form between a lysine residue of one ubiquitin molecule and the C-terminal glycine residue of another ubiquitin molecule. Each human ubiquitin monomer is 76 amino acids (aa) in length and shares 96% and 100% aa identity with yeast and mouse ubiquitin, respectively. Seven of the 76 aa in ubiquitin are lysine residues that can participate in poly-ubiquitin chain formation. Linkage through specific lysine residues is thought to serve as a signal that affects protein degradation, signaling, trafficking and other cellular processes.

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