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Kit

Quantitative ELISA

Specific to Fe2+ and Fe3+.

All

Enough reagents for 100 tests.

10 - 1000μg/dL

Cell LysatePlasmaSalivaSerumTissue SupernatantUrine

Colorimetric

1 x 16 ml R-1 Buffer (ready to use)1 x 7 ml R-2 Buffer (ready to use)1 x 0.7 ml R-3 Chelate color (ready to use) 1 x 1.5 ml STD Iron (Fe) standard 200 µg/dL (ready to use)

This MC-Reagent Iron Assay Kit is a direct colorimetric assay based on the Nitroso-PSAP method without deproteinization of the sample. A weak acid buffer dissociates iron from the transferrin-iron complex and is reduced by a reductant (Ferric >> Ferrous). Ferrous ions form a complex with the chromogen Nitroso-PSAP. The color intensity is proportional to the iron concentration in the sample. Absorbance of the Fe2+-complex is measured at 750nm. Wavelength range of sensitivity: 740 ~ 760nm.Features of this Assay: Quick & Easy to use • Species independent • Highly sensitive, stable and suitable for high-throughput testing • No toxic substances • For cell lysates, serum, plasma and wide variety of biological samples

Manufactured by JaICA.

BLUE ICE

+4°C

+4°C

Do not freeze.

12 months after the day of manufacturing. See expiry date on ELISA Kit box.

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Iron is a mineral (functioning as an enzyme cofactor) that plays an essential role in many biological processes. It is essential to nearly all known organisms. As a transition element it can form a range of oxidation states, the most common being Fe²⁺ (or ferrous iron) and Fe³⁺ (or ferric iron). Ingested iron is mainly absorbed in the form of Fe²⁺. The trivalent form and the heme-bound Fe²⁺-component of iron is reduced by vitamin C. Before passing into the plasma, it is oxidized by ceruloplasmin to Fe³⁺ and bound to transferrin to form a transferrin-iron complex. Iron is generally stored in the centre of metalloproteins, in the heme complex, and in oxygen carrier proteins. Iron-containing proteins participate in many reactions, often utilizing transitory changes in the oxidation state of iron to carry out chemical reactions. Iron is important for redox reactions, oxygen transport (e.g. hemoglobin), short-term oxygen storage (e.g. myoglobin) and energy generation. Iron deficiency has many adverse consequences, including anemia, hemochromatosis, chronic renal disease and in children, behavioral and learning disorders. Iron excess is toxic to the body, harming the heart, liver, skin, pancreatic islet beta cells, bones, joints, and pituitary gland. Maintaining proper iron balance is essential for maintaining homeostasis and health.

Product References

• An automated method for measurement of serum iron and unsatur- ated iron binding capacity using nitroso-PSAP: M. Yamada, et al.; Jpn. J. Lab. Auto. 13, p659 (1988)
• FecA1, a bacterial iron transporter, determines the survival of Helicobacter pylori in the stomach: H. Tsugawa, et al.; Free Radic. Biol. Med. 52, 1003 (2012)
• Estrogen Regulates Hepcidin Expression via GPR30-BMP6-Dependent Signaling in Hepatocytes: Y. Ikeda, et al.; PLoS One 7, e40465 (2012)
• GRK6 deficiency in mice causes autoimmune disease due to impaired apoptotic cell clearance; M. Nakaya; Nat. Comm. 4, 1532 (2013)
• Superparamagnetic nanoparticle clusters for cancer theranostics combining magnetic resonance imaging and hyperthermia treatment: K. Hayashi, et al.; Theranostics 3, 366 (2013)

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