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Interleukin-33; IL-1F11; NF-HEV

Polyclonal Antibody

Rabbit

Recombinant mouse IL-33.

ELISA: (direct and indirect: 1:2’000-1:5’000)Western Blot: (1:2’000-1:5’000 using ECL; suggested blocking and dilution buffer is PBST containing 0.05% Tween 20 and 5% skim milk; suggested incubation time is 1 hour at room temperature).Optimal conditions must be determined individually for each application.

Mouse

Recognizes mouse IL-33. Detects a band of ~18kDa by Western blot. Does not cross-react with human IL-33.

Protein A-affinity purified.

1mg/ml

Liquid. 0.2μm-filtered solution in PBS, pH 7.4. Contains no preservatives.

BLUE ICE

+4°C

-20°C

After opening, prepare aliquots and store at -20°C.Avoid freeze/thaw cycles.

Stable for at least 6 months after receipt when stored at -20°C.

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Interleukin-33 (IL-33; HF-NEV; IL-1F11), a member of the IL-1 family of cytokines, is expressed by many cell types following pro-inflammatory stimulation and is thought to be released on cell lysis. The 30kDa human IL33 is converted by CASP1 to a 18kDa protein. IL33 binds to and signals through ST2 (IL1R1) and its stimulation recruits MYD88, IRAK, IRAK4, and TRAF6, followed by phosphorylation of ERK1(MAPK3)/ERK2(MAPK1), p38(MAPK14), and JNK. The ability of IL-33 to target numerous immune cell types, like Th2-like cells, mast cells, and B1 cells, and to induce cytokine and chemokine production underlines its potential in influencing the outcome of a wide range of diseases, such as arthritis, asthma, atopic allergy & anaphylaxis, cardiovascular disease/atherosclerosis, nervous system diseases, and sepsis.Product References

Soluble ST2 blocks interleukin-33 signaling in allergic airway inflammation: H. Hayakawa, et al.; JBC 282, 26369 (2007)

A novel method for procuring a large quantity of mature murine eosinophils in vivo: W. Kim, et al.; J. Immunol. Methods 363, 90 (2010)

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