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Protein

HeLa cells

Human

Liquid. In HEPES.

Use: This Protein Fraction I can be used to demonstrate F1 protein-dependent substrate degradation or conjugation. Reaction conditions will need to be optimized for each specific application. We recommend an initial concentration of 0.5-4mg/mL. Additionally, we recommend the inclusion of an isopeptidase inhibitor and a proteasome inhibitor when investigating substrate conjugation.

Manufactured by Boston Biochem

DRY ICE

-20°C

-80°C

Aliquot to avoid freeze/thaw cycles.

Stable for at least 6 months after receipt when stored at -80°C.

No

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Protein fraction of a cell extract that does not bind to anion exchange resin. Contains ubiquitin and fraction I E2 and E3s enzymes. FI is essentially free of ATP.

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