品牌咨询
联系方式
公司地址
苏州工业园区生物纳米园A4#216
联系电话
4000-520-616 / 18915418616
传真号码
0512-67156496
电子邮箱
info@ebiomall.com
公司网址
https://www.ebiomall.com
商品描述
ProductDetails | |
---|---|
ProductType | Protein |
Properties | |
Source/Host | HeLacells |
Crossreactivity | Human |
Application | WesternBlot |
Concentration | 1mg/ml |
OtherProductData | ClickhereforOriginalManufacturerProductDatasheet Ourproductdescriptionmaydifferslightlyfromtheoriginalmanufacturersproductdatasheet. Boilfor5minutestoreducethepreparation.Load20ugofreducedlysateperlaneforImmunoblotanalysis. |
Declaration | ManufacturedbyAbFrontier |
ShippingandHandling | |
Shipping | BLUEICE |
ShortTermStorage | +4°C |
LongTermStorage | -20°C |
HandlingAdvice | Avoidfreeze/thawcycles. |
Use/StABIlity | Stableforatleast6monthsafterreceiptwhenstoredat-20°C. |
Documents | |
MSDS | No |
ProductSpecificationSheet | |
Datasheet | DownloadPDF |
HeLa(Humanepithelialcarcinomacellline)WholeCellLysate.CellswerelysedinLysisbuffer(20mMHEPES,pH7.2,20mMPhosphoglycerate,150mMNaCl,10%glycerol,1%NP-40,1mMEDTA,1mMEGTA,1mMNa3VO4,5mMNaF,1mMPMSF,5ug/mlPepstatinA,5ug/mlAprotinin,5ug/mlLeupeptin)anddilutedwithreducingsamplebuffer(60mMTris,pH6.8,6%glycerol,2%SDS,0.2%bromphenolblue,200mM2-mercaptoethanol).