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蚂蚁淘在线 / 品牌中心 / Adipogen / Adipogen/RAP80 (UIM domains) (human) (rec.) (Biotin)/AG-40T-0349B-C250/250 µg
商品描述
| ProductDetails | |
|---|---|
| Synonyms | ReceptorAssociatedProtein80;RXRIP110;UIMC1;X2HRIP110;BRCA1-AComplexSubunitRAP80;RetinoidXReceptor-interactingProtein110;UbiquitinInteractionMotif-containingProtein1 |
| ProductType | Protein |
| Properties | |
| Source/Host | E.coli |
| Sequence | HumanRAP80(UIMdomainsaa79-124)(AccessionNr.NP_057374)conjugatedtobiotin. |
| Crossreactivity | Human |
| Label/Conjugates | Biotin |
| Formulation | Liquid.In50mMHEPES,250mMNaCl,1mMDTT,5%GlycerolpH8.0. |
| OtherProductData | Use:Use50-100µgofproteintodetect10-20µgofpurifiedK63-linkedubiquitinchains.Theamountnecessaryforuseincrudelysatesneedstobedeterminedempirically.Detectionwithavidin-linkedreagentsallowsforahigherefficiencyanddetectionsensitivitythanwithotherantibodies. |
| Declaration | ManufacturedbyBostonBiochem |
| ShippingandHandling | |
| Shipping | DRYICE |
| ShortTermStorage | -20°C |
| LongTermStorage | -80°C |
| HandlingAdvice | Aliquottoavoidfreeze/thawcycles. |
| Use/StABIlity | Stableforatleast1yearafterreceiptwhenstoredat-80°C. |
| Documents | |
| MSDS | No |
| ProductSpecificationSheet | |
| Datasheet |  DownloadPDF |
Rap80(ReceptorAssociatedProtein80)interactswithBRCA1,aubiquitinE3-ligasewhichfunctionsinconjugationwiththeBARD1deubiquitinatingenzyme.BRCA1isrecruitedtoDNAdamagesitesbypoly-ubiquitinchainsthroughRap80whichcontains2tandemubiquitin-interactingmotifs(UIMs).Rap80constitutesaproteincomplexwithABRA1whichinteractswiththeBRCTdomainofBRCA1.UponDNAdamagetheRap80-ABRA1complextargetstheBRCA1-BARD1complextoK6-andK63-linkedpoly-ubiquitinchainsatthesefoci.TheUIMdomainsofRap80havebeenshowntohavepreferentialbindingtoK-6andK63-linkedubiquitinchainsandbindstoK48-chainwithamuchlowerefficiency.ItisnotknownifRap80UIMsinteractwithubiquitinchainslinkedviaK11,K27orK33.Rap80hasalowaffinityformono-,di-andtri-ubiquitinbutbindsefficientlytotetra(orgreater)ubiquitinchains.
