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4000-520-616 / 18915418616
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0512-67156496
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商品描述
| ProductDetails | |
|---|---|
| Synonyms | MultimericCD40L™;ACRP30headless:CD40L;ACRP30headless:CD154;ACRP30headless:TNFSF5;ADIPOQ-CD40L |
| ProductType | Protein |
| Properties | |
| Source/Host | CHOcells |
| Sequence | RatCD40L(aa115-260)isfusedattheN-terminustomouseACRP30headless(aa18-111)andaFLAG®-tag. |
| Crossreactivity | Human Mouse Rat |
| Specificity | Bindstorat,humanandmouseCD40. |
| BIOLOGicalActivity | InducesBcellactivation(asdemonstratedbydose-dependentupregulationofCD86). |
| MW | ~35-40kDa |
| Purity | ≥95%(SDS-PAGE) |
| EndotoxinContent | <0.02eu mu;g="" purified="" protein="" (lal="" test;="" lonza).=""> |
| Concentration | 0.1mg/mlafterreconstitution. |
| Reconstitution | Reconstitutewith100μlsterilewater. |
| Formulation | Lyophilized.ContainsPBS. |
| OtherProductData | UniprotlinkQ9Z2V2:CD40L(rat) FLAGisaregisteredtrademarkofSigma-AldrichCo. |
| ShippingandHandling | |
| Shipping | BLUEICE |
| ShortTermStorage | +4°C |
| LongTermStorage | -20°C |
| HandlingAdvice | Afterreconstitution,preparealiquotsandstoreat-20°C. Avoidfreeze/thawcycles. PBScontainingatleast0.1%BSAshouldbeusedforfurtherdilutions. |
| Use/StABIlity | Stableforatleast6monthsafterreceiptwhenstoredat-20°C. |
| Documents | |
| MSDS |  DownloadPDF |
| ProductSpecificationSheet | |
| Datasheet | DownloadPDF |
MultimericCD40L™isahighactivityconstructinwhichtwotrimericCD40ligandsareartificiallylinkedviathecollagendomainofACRP30.Thisconstructveryeffectivelysimulatesthenaturalmembrane-assistedaggregationofCD40Linvivo.ItprovidesasimpleandequallypotentalternativetoCD40L+enhancercombinations.MultimericCD40L™hasbeenshowntosuppressalum-inducedIL-1βreleaseandcaspase-1activationinadose-,CD40-andtimedependentmanner,withoutaffectingBMDM(Bonemarrow-derivedmacrophages)viability.ItalsoeffectivelysuppressedtheinflammasomefunctiontriggeredbyNLRP3activators.Thesecretionofcaspase-1independentinflammatorymediatorshasbeenshowntobeunalteredorevenenhanced.
