Known in its anhydrous form as Coenzyme II, this substance is a fundamental metabolite studied in nucleotide chemistry. Nicotinamide dinucleotide phosphate (NADP) is a coenzyme carrying electrons and hydrogen in a multitude of reactions. Forms include oxidized NADP+ and reduced NADPH, a product of the pentose phosphate pathway (PPP). The molecule acts as an electron acceptor and the conjugate acid to NADP zwitterion.

NADP functions in electron transfer in the anabolic biosynthesis of lipids, cholesterol and fatty acyl chains. The substance can therefore be applied to antioxidant roles preventing oxidative damage. NADP+ and NADPH, paired together for redox reactions, participate in enzyme oxidation-reduction catalysis. NADP is further implicated in metabolism, mitochondrial conditions and apoptosis-induction.

Ferredoxin-NADP+ reductase produces NADPH in photosynthesis and is the reducing agent in the Calvin cycle, transforming CO₂ into glucose for energy. NADP further reduces nitrate in the nitrogen cycle, providing the plant with ammonia. In animals, it contributes to the creation of NADPH and ADP. Reduction powers of NADPH protect these organisms from reactive oxygen species (ROS).

Product SpecificationsCoenzyme IIb-NADPNAPD+beta-Nicotinamide adenine dinucleotide phosphate hydrateNicotinamide adenine dinucleotide phosphateTriphosphopyridine nucleotide

Formula: C₂₁H₂₈N₇O₁₇P₃(anhydrous)

MW: 743.40 g/mol (anhydrous)

Storage/Handling: Store desiccated at 4°C. Protect from light and air.

PubChem Chemical ID: 5885

★活体技术荧光检测荧光蛋白标记适用于标记细胞、病毒、基因等，通常使用的是 GFP、EGFP、RFP（DsRed）等；荧光染料标记适用于标记抗体、多肽、小分子药物等；常用的有 Cy3、Cy5、Cy5.5 及 Cy7量子点标记（新的标记方法）适用于标记肿瘤，主要应用在活细胞实时动态荧光观察与成像；量子点（quantum dot) 是一种能发射荧光的半导体纳米微晶体，外观恰似一极小的点状物。量子点荧光比有机荧光染料的发射光强的 20 倍，稳定性强 100 倍以上，具有荧光发光光谱较窄、量子产率高、不易漂白、激发光谱宽、颜色可调，并且光化学稳定性高，不易分解等诸多优点。