We have reengineered our GoldBio Simple Stop™ 2 to have significantly better protection against Acid Phosphatases as well as enhanced protection against Protein Phosphatase. Additionally, Simple Stop™ 2 contains a higher concentration of activated Sodium Orthovanadate (compared to Simple Stop™ 1).

Simple Stop™ 2 is a broad-spectrum phosphatase inhibitor cocktail consisting of five phosphatase inhibitors in two solutions that target all the phosphatase categories: serine/threonine (Ser/Thr) specific phosphatase, tyrosine specific phosphatase, dual specificity phosphatases, acid phosphatase and alkaline phosphatase.

Each Simple Stop™ phosphatase inhibitor cocktail solution is a 100X concentrated, ready-to-use, solution. Simply add cocktail solution “A” and cocktail solution “B” to your extraction buffers or samples and you are ready to go. This cocktail is ideal for inhibition in tissue extractions and cell lysis experiments and is compatible with most common protein assays.

Kit Components

• Cocktail solution A (1 mL): 0.25M Sodium Fluoride, 0.1M Sodium Pyrophosphate and 0.1M activated Sodium Orthovanadate
• Cocktail solution B (1 mL): 100mM β-glycerophosphate and 1M Sodium Molybdate

Storage/Handling: Store at 4°C.Precipitate may occasionally occur in the solution. To solubilize, heat at 37-48°C for five minute and vortex.

★活体技术荧光检测荧光蛋白标记适用于标记细胞、病毒、基因等，通常使用的是 GFP、EGFP、RFP（DsRed）等；荧光染料标记适用于标记抗体、多肽、小分子药物等；常用的有 Cy3、Cy5、Cy5.5 及 Cy7量子点标记（新的标记方法）适用于标记肿瘤，主要应用在活细胞实时动态荧光观察与成像；量子点（quantum dot) 是一种能发射荧光的半导体纳米微晶体，外观恰似一极小的点状物。量子点荧光比有机荧光染料的发射光强的 20 倍，稳定性强 100 倍以上，具有荧光发光光谱较窄、量子产率高、不易漂白、激发光谱宽、颜色可调，并且光化学稳定性高，不易分解等诸多优点。