Description

T4 DNA Polymerase has both a DNA-dependent DNA polymerase activity and a potent 3´→5´ exonuclease activity. T4 DNA Polymerase is idea for removal of 3´-overhangs or filling in 5´-overhang to form blunt ends, ligation-independent cloning of PCR products, or second strand synthesis in site-directed mutagenesis. Our T4 polymerase is proven to be free from detectable endonuclease and RNase activities.

Product Includes

• T4 DNA Polymerase
• 10x T4 DNA Polymerase Reaction Buffer

Purity: ≥95% (assessed by SDS-PAGE with Coomassie blue staining)

Concentration: 5 unit/μl

One unit of T4 DNA Polymerase converts 10 nmol of dNTPs into acid-insoluble material in 30 minutes at 37°C under standard assay conditions.

Source: E. coli cells with a cloned gene of bacteriophage T4 DNA Polymerase

Inactivation: Inactivated by heating at 70°C for 15 minutes.

Storage/Handling: Store at -20°C. Product may be shipped on blue ice.

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