Cobalt HTC 6% Agarose Beads are suitable for downstream purification using IMAC. These resins combine the advantages of Cobalt purification with the high flow rates of the HTC resin (70% higher than commercial standards).
The maximum flow rate at 15 cm bed height is >1000 cm/h and the maximum pressure is >300 kPa.The binding capacity with an approximately 30 kDA protein is 5 mg/mL.
These products are excellent for large scale His-tagged protein purifications:
- Easy scale up and robust function
- High chemical and physical stabilities
- Good resolution in minimum time
HTC beads are high throughput beads designed to meet the demand for industrial process separation.Their rigidity and mechanical resistance permits high flow rates with good resolution in a minimum time frame, making these beads ideal for process-scale use.HTC beads are highly crosslinked 4% and 6% agarose matrices and have excellent physical and chromatographic qualities.They are autoclavable.These beads are an ideal support for the immobilization of ligands for Affinity Chromatography and base media support for producing IEX (Ion Exchange) and hydrophobic interaction chromatography resins.
Storage/Handling: Store at 4°C. Do not freeze.
★活体技术荧光检测荧光蛋白标记适用于标记细胞、病毒、基因等,通常使用的是 GFP、EGFP、RFP(DsRed)等;荧光染料标记适用于标记抗体、多肽、小分子药物等;常用的有 Cy3、Cy5、Cy5.5 及 Cy7量子点标记(新的标记方法)适用于标记肿瘤,主要应用在活细胞实时动态荧光观察与成像;量子点(quantum dot) 是一种能发射荧光的半导体纳米微晶体,外观恰似一极小的点状物。量子点荧光比有机荧光染料的发射光强的 20 倍,稳定性强 100 倍以上,具有荧光发光光谱较窄、量子产率高、不易漂白、激发光谱宽、颜色可调,并且光化学稳定性高,不易分解等诸多优点。