The Yeast Spheroplast Lysis Buffer is useful for extraction of soluble proteinsfrom spheroplast cells. It is a proprietary improvement on the Zymolyase®based spheroplast preparation and extraction of soluble proteins fromyeast cells.

Yeast Lysis Buffer is based on organic buffering agents that utilize a mild non-ionic detergent and a proprietary combination of varioussaltsand agents to enhance extraction and stability of proteins. Depending on application, additional agents such as reducing agents,chelating agents, and protease inhibitors may be added into Yeast LysisBuffer. The Yeast Lysis Buffer has been optimized with Saccharomyces cerevisiae and has been tested on several widely used yeast strains.It also works well with both gram-positive and gram-negative bacteria.

The proprietary combination of this reagent provides a simple andversatile method of yeast protein extraction. Yeast Lysis Buffereliminates the need for laborious glass bead lysis of yeast cells.

Storage/Handling: Store at -20°C. When stored under the recommended conditions and handled correctly, these products should be stable for at least 1 year from the date of receipt.

★活体技术荧光检测荧光蛋白标记适用于标记细胞、病毒、基因等，通常使用的是 GFP、EGFP、RFP（DsRed）等；荧光染料标记适用于标记抗体、多肽、小分子药物等；常用的有 Cy3、Cy5、Cy5.5 及 Cy7量子点标记（新的标记方法）适用于标记肿瘤，主要应用在活细胞实时动态荧光观察与成像；量子点（quantum dot) 是一种能发射荧光的半导体纳米微晶体，外观恰似一极小的点状物。量子点荧光比有机荧光染料的发射光强的 20 倍，稳定性强 100 倍以上，具有荧光发光光谱较窄、量子产率高、不易漂白、激发光谱宽、颜色可调，并且光化学稳定性高，不易分解等诸多优点。