GelRed™isanultra-sensitive,extremelystableandenvironmentallysafefluorescentnucleicaciddyedesignedtoreplacethehighlytoxicethidiumbromide(EtBr).6XGelRed™PrestainLoADIngBuffersaregelloadingbufferscontainingdensityagents,trackingdyes,andGelReddye.The6XprestainloadingbufferisaddedtosamplesinplaceofgelloadingbufferandeliminatestheneedtoaddfluorescentDNAdyetotheagarosegelduringcasting.

6XGelRed™PrestainLoadingBufferwithBlueTrackingDyescontainstwoblueelectrophoresistrackingdyesthatrunatapproximately1.5kband200bpina1%agarosegel.Forsmallertrackingdyes,seeCatalog#G-735(6XGelRed™PrestainLoadingBufferwithOrangeTrackingDyes).

GelRed™andEtBrhavevirtuallythesamespectra,soyoucandirectlyreplaceEtBrwithGelRedwithoutchangingyourexistingimagingsystem.Inaddition,GelRed™isfarmoresensitivethanEtBr,whichcannotbeusedinDNAloadingbuffertoprestainDNA.GelRed™iscompatIBLewithdownstreamapplicationssuchassequencingandcloning.GelRed™isefficientlyremovedfromDNAbycommercialgelextractionkitsorbyphenol/chloroformextractionandethanolprecipitation.

AseriesofsafetytestshaveconfirmedthatGelRed™isnoncytotoxic,nonmutagenicandnonhazardousatconcentrationswellabovetheworkingconcentrationsusedingelstaining.Asaresult,GelRed™canbesafelydisposedofdownthedrainorinregulartrash,providingconvenienceandreducingcostinwastedisposal.

Features

SaferthanEthidumBromide	ShownbytheAmestestandotherteststobenonmutagenicandnoncytotoxic
Easydisposal	Passedenvironmentalsafetytestsfordirectdisposaldownthedrainorinregulartrash
Ultra-sensitive	HighersensitivitythanEtBrandSYBR®Safe
Extremelystable	Availableinwater,stableatroomtemperatureforlong-termstorageandmicrowavable
Simpletouse	Verysimpleproceduresforprecastorpost-electrophoresisgelstaining
CompatiblewithastandardUVtransIlluminator	ReplacesEtBrwithnoopticalsettingchange
Compatiblewithdownstreamapplications	Compatiblewithgelpurification,restrictiondigest,sequencingandcloning

Storage/Handling:Storeat4°C.Protectfromlight.

Productmaybeshippedatambienttemperaturewithoutreducingperformance.Pleaseplaceattherecommendedstorageconditionsuponreceiptoftheproduct.

GelRedanditsusesarecoveredbyUSpatentnumbers7960498,7803943,and8232050.SYBRistrademarkofMolecularprobes,Inc.

★活体技术荧光检测荧光蛋白标记适用于标记细胞、病毒、基因等，通常使用的是 GFP、EGFP、RFP（DsRed）等；荧光染料标记适用于标记抗体、多肽、小分子药物等；常用的有 Cy3、Cy5、Cy5.5 及 Cy7量子点标记（新的标记方法）适用于标记肿瘤，主要应用在活细胞实时动态荧光观察与成像；量子点（quantum dot) 是一种能发射荧光的半导体纳米微晶体，外观恰似一极小的点状物。量子点荧光比有机荧光染料的发射光强的 20 倍，稳定性强 100 倍以上，具有荧光发光光谱较窄、量子产率高、不易漂白、激发光谱宽、颜色可调，并且光化学稳定性高，不易分解等诸多优点。