TaqDNAPolymeraseisaThermostableDNApolymerasethatpossessesa5´→3´polymeraseactivity(1,2)anda5´flapendonucleaseactivity(3,4).TaqpolymeraseisidealforprimaryextensionreactionwithDNAfragmentshavingdAoverhangon3’ends.Thisproductissuppliedwitha10xPCRreactionbufferthatprovidesafinalconcentrationof1.5mMMg²⁺andanoptional5XGCEnhancerthatincreasestheamplificationofGCrichtemplatesupto84%.The10xbufferalsoincludesaninertbluedyeforvisIBLetrackingandthepreventionofpipettingmistakes,wastedtime,reagentsandmoney.

ProductIncludes

• TaqDNAPolymerase
• 10xPCRBufferwithMg²⁺withconveniencedye
• 5xGCEnhancer

Purity:≥98%(assessedbySDS-PAGEwithCoomassiebluestaining)

Concentration:1unit/μlOneunitisdefinedastheamountofenzymethatincorporates10nmolesofdNTPintoacid-insolubleformin30minutesat72ºC.

Source:E.colistrainexpressingaTaqDNAPolymerasegenefromThermusaquaticusYT-1

Storage/Handling:Storeat-20°C.Productmaybeshippedonblueice.

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