Skeletalmuscleisanimportantsiteofinsulin-stimulatedglucosedisposalandoftenthesiteofinsulinresistanceinobesity.Humanprimaryculturedskeletalmyoblastscandirectlyreflectapatient"smetabolicphenotype,becausemanyofthesignalingpathwaysaremaintainedintact.Zenbiooffershumanprimaryskeletalmyoblastsfromavarietyofdonors,includingobesedonorswithType2diabetes.
HumanskeletalmyoblastssatellitecellsareisolatedfromconsentedpatientswithgoodrepresentationfromalllevelsofADIposityandage.Skeletalmyoblastsareavailablefrombothmaleandfemaledonors.PooledlotsofcellsareavailabletoprovidelargelotsforscreeningandtodecreaseissueswithpatienttopatientvariABIlity.
Cellsareavailablecryopreserved(500,000cells/vial).
QUALITYCONTROL:Allcellshavebeentestedformyotubedifferentiation.CellshavetestednegativeforHIV-1,HIV-2,HTLC-1,HTLV-2,HepatitisB,HepatitisCandmycoplasma.HumanskeletalmyoblastaredifferentiatedusingSkeletalMuscleDifferentiationMedium(cat#SKM-D)foratleast6days.Myogenicdifferentiationwasverifiedbyimmunohistochemicalstainingusingantibodiestodesminandmyosin.Thedifferentiatedmyocytesalsorespondtoinsulinstimulatedglucoseuptake.
Speciallyformulatedmediaareavailableformaintenanceanddifferentiationofouradultskeletalmyoblasts.
