ZYMO RESEARCH/ZR-96 DNA Clean-Up Kit/4 x 96 Preps/D4018

价格
¥8400.00
货号:D4018
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品牌:ZYMO RESEARCH
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商品描述
Clean-up PCR and other enzymatic reactions in as little as 2 minutes
Highlights

  • Quick (20 minute), large-scale recovery of ultra-pure DNA from PCR, endonuclease digestions, cell-free lysates, etc.
  • ZR-96 Silicon-A Plate design allows DNA to be eluted at high concentrations into minimal volumes of solvent.
  • Eluted DNA is well suited for use in PCR, DNA sequencing, DNA ligation, endonuclease digestion, RNA transcription, radiolabeling, etc.
Description

The ZR-96 DNA Clean-up Kit is a PCR purification kit that provides for rapid, large-scale (96-well) purification and concentration of high-quality DNA from PCR samples, endonuclease digestions, or crude plasmid preparations. Simply add the specially formulated DNA Binding Buffer to your samples and transfer to the wells of the supplied Silicon-A Plate. There is no need for organic denaturants or chloroform. Instead, the product features Fast-Spin plate technology to yield high-quality, purified DNA in just minutes.


Detergent Tolerance≤5% Triton X-100, ≤5% Tween-20, ≤5% Sarkosyl, ≤0.1% SDS
Elution Volume≥ 30 µl for shallow well, ≥ 10 µl for deep well
EquipmentCentrifuge with microplate carriers
PurityA260/A280 > 1.8
Sample Source DNA from PCR, endonuclease digestions, DNA modification reactions, isotope/fluorescence labeling reactions, etc.
Size Range75 bp to 23 kb for shallow well, 50 bp to 23 kb for deep well
Yield≤ 5 µg total DNA can be recovered.For DNA 75 bp to 10 kb the recovery is 70-90%. For DNA 11 kb to 23 kb the recovery is 50-70%.

Q1: What is the lower limit and minimal amount of DNA that can be recovered?

Picogram levels of DNA can be recovered. The limitation is based on sensitivity of detection method.

Q2: How to process naked DNA stored in DNA/RNA Shield?

Add an equal volume of ethanol (95-100%) to the sample and mix well. The sample is ready-to-bind and does not require DNA Binding Buffer. Proceed to Step 2.

Q3: What to do if ethanol addition to the DNA Wash Buffer was omitted?

The DNA will be eluted off the column. Rebind samples using the appropriate amount of DNA Binding Buffer and wash the column with the properly prepared wash buffer.

Q4: What happens if more DNA was loaded on the columns than the stated maximum binding capacity?

Oversaturation of the column can result in total DNA loss due to clogging of silica matrix.

Q5: How many times can columns be reloaded?

We recommend no more than 5 times as binding efficiency might decrease.

Q6: What is the minimum input volume of DNA sample?

Working with volumes below 50 µl can result in decreased recovery. We recommend raising the starting volume to 100 µl with water to ensure optimal binding conditions.



Cat #NameSizePrice
D4003-2-24DNA Wash Buffer (Concentrate)24 ml$33.00
D4004-1-LDNA Binding Buffer100 ml$57.00
D4003-2-48DNA Wash Buffer (Concentrate)48 ml$60.00
C2001Silicon-A Plate2 Plates$129.00
C2003Elution Plate2 Plates$19.00
C2002Collection Plate2 Plates$22.00
ZYMO RESEARCH的DNA / RNA屏蔽DNA / RNA Shield是任何生物样品的DNA和RNA传输和存储介质。DNA / RNA Shield可在环境温度下(无需冷藏或冷冻)保留样品的遗传完整性和表达特征,并完全灭活传染性因子(病毒,细菌,真菌和寄生虫)。可以直接从存储在该DNA和RNA传输和存储介质中的样品中分离核酸,而无需沉淀或去除试剂(与大多数DNA和RNA纯化试剂盒兼容)。