ZYMO RESEARCH/OneStep PCR Inhibitor Removal Kit/50 Preps/D6035

价格
¥6500.00
货号:D6035
浏览量:127
品牌:ZYMO RESEARCH
服务
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商品描述
Removal of potent PCR inhibitors in one step
Highlights

  • Removes PCR inhibitors such as polyphenolics, humic/fulvic acids, tannins, melanin, etc. from nucleic acid solutions to yield high quality DNA or RNA.
  • Fast, one-step procedure for cleaning impure samples prior to PCR, sequencing, reverse transcription (RT), etc.
Description

The OneStep PCR Inhibitor Removal Kits are PCR inhibitor clean up kits that contain all the components needed for efficient removal of contaminants that can inhibit downstream enzymatic reactions (e.g. PCR and RT) from DNA and RNA preparations. The column matrices have been specifically designed for the efficient removal of polyphenolic compounds, humic/fulvic acids, tannins, melanin, etc. from the most impure DNA and RNA preparations. Sample clean-up is as simple as applying, spinning, and recovering a sample from the column.


Applicable ForCleaned samples are ready for PCR, sequencing, reverse transcription (RT), etc.
Elution Volume50 - 200 µl
EquipmentMicrocentrifuge
PurityHigh quality, enzymatic-reaction ready DNA/RNA products.
Sample SourceDNA/RNA samples contaminated with enzymatic reaction inhibitors.
Sample StorageEluted DNA/RNA can be used immediately or stored at ≤ -20°C.
TypeDNA and RNA
Yield50 - 90% recovery

Q1: What is the smallest input volume of DNA that can be added to the OneStep Inhibitor Removal kit?

The smallest volume will be 50 ul.

Q2: What is the largest input volume of DNA that can be added to the OneStep Inhibitor Removal kit?

The OneStep PCR Inhibitor Removal Kit can process about 500 µl of sample at a time. However, the same column can be reused for the same sample.

Q3: Can I centrifuge at a lower speed than 3,500 x g for the 96-well kit?

No, the kit requires the speed to be at 3,500 x g. Centrifuging at lower speeds can result in inefficient sample elution.



“The ease.Holy cow, this thing was so freaking simple compared to similar products”

-Christian H. (University of Georgia)

“It was VERY fast and yielded the intended results...PCR products from previously non-amplifiable DNA.”

-Tara N. (United States Agricultural Research Service)

Cat #NameSizePrice
C1001-50Collection Tubes50 Pack$15.00
D6035-1-30Prep Solution30 ml$18.00
C1058-50Zymo-Spin III-HRC Filters50 Pack$108.00
C2003Elution Plate2 Plates$19.00
C2009Silicon-A-HRC Plate2 Plates$405.00
ZYMO RESEARCH的DNA / RNA屏蔽DNA / RNA Shield是任何生物样品的DNA和RNA传输和存储介质。DNA / RNA Shield可在环境温度下(无需冷藏或冷冻)保留样品的遗传完整性和表达特征,并完全灭活传染性因子(病毒,细菌,真菌和寄生虫)。可以直接从存储在该DNA和RNA传输和存储介质中的样品中分离核酸,而无需沉淀或去除试剂(与大多数DNA和RNA纯化试剂盒兼容)。