ZYMO RESEARCH/DNA Clean & Concentrator-500/20 Preps/D4031

价格
¥2760.00
货号:D4031
浏览量:127
品牌:ZYMO RESEARCH
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商品描述
Clean-up PCR and other enzymatic reactions in as little as 2 minutes
Highlights

  • Simple, rapid recovery of ultra-pure DNA from PCR, endonuclease digestions, and cell-free DNA preps, etc.
  • Unique column construction allows sample loading and washing to be performed using a centrifuge, microcentrifuge, or vacuum source.
  • Eluted DNA is well suited for use in PCR, DNA sequencing, DNA ligation, endonuclease digestion, RNA transcription, radiolabeling, arrays, etc.
Description

The DNA Clean & Concentrator-500 (DCC-500) is our highest capacity PCR purification kit of the DNA Clean & Concentrator products. It is designed for the rapid, large format purification and concentration of up to 500 µg of high quality DNA from samples such as large-scale restriction endonuclease digestions and crude DNA preparations. Eluted DNA is well suited for use in PCR, DNA sequencing, DNA transfection, DNA ligation, endonuclease digestion, RNA transcription, radiolabeling, etc. The entire DNA purification/concentration procedure typically takes less than 20 minutes.


Detergent Tolerance≤5% Triton X-100, ≤5% Tween-20, ≤5% Sarkosyl, ≤0.1% SDS
Elution Volume≥ 2 ml
EquipmentMicrocentrifuge and centrifuge or vacuum source.
PurityHighly purified DNA is eluted with water and is especially well suited for sequencing, ligation reactions, and restriction endonuclease digestions.
Sample SourceDNA from large-scale sample sources including restriction endonuclease digestions and "crude" DNA preparations from cell-free lysates.
Size Range50 bp to 23 kb
Yield≤ 500 µg total DNA can be recovered.For DNA 50 bp to 10 kb the recovery is 70-95%. For DNA 11 kb to 23 kb the recovery is 50-70%.

Q1: What is the minimum input volume of DNA sample?

Working with volumes below 50 µl can result in decreased recovery. We recommend raising the starting volume to 100 µl with water to ensure optimal binding conditions.

Q2: What happens if more DNA was loaded on the columns than the stated maximum binding capacity?

Oversaturation of the column can result in total DNA loss due to clogging of silica matrix.

Q3: How many times can columns be reloaded?

We recommend no more than 5 times as binding efficiency might decrease.

Q4: How to process naked DNA stored in DNA/RNA Shield?

Use the standard protocol (add 2 or 5 volumes of DNA binding buffer depending on DNA size.

Q5: What is the lower limit and minimal amount of DNA that can be recovered?

Picogram levels of DNA can be recovered. The limitation is based on sensitivity of detection method.

Q6: What to do if ethanol addition to the DNA Wash Buffer was omitted?

The DNA will be eluted off the column. Rebind samples using the appropriate amount of DNA Binding Buffer and wash the column with the properly prepared wash buffer.




“I sampled this kit next to a kit we already use. I had three of the same sample, two for the Zymo kit and one for the other. On one of the ones designated for your kit, I accidentally added the sample directly to the column filter, then added other reagents (I figured I might as well try it). The other I did as per the instructions. The one on which I goofed gave a similar purification and concentration as the other kit, while the one I did properly yielded approximately four times the DNA per µL as the other kit. I am very happy with this result, and when I rerun my samples, I plan to use your product.”

-Rosalind P. (University of Arkansas for Medical Sciences)

“Almost no loss of elution buffer at the final step. It is quite impressive compared to the other kits I have used, which lose about 5-7µl.”

-Joseph R. (Miller School of Medicine, University of Miami)

“It was a very simple procedure and it gave a concentration ten times the original amount.”

-Kimberly M. (University of Pennsylvania)
Read More

Cat #NameSizePrice
D3004-4-16DNA Elution Buffer16 ml$18.00
D3004-4-50DNA Elution Buffer50 ml$32.00
D4003-1-LDNA Binding Buffer50 ml$33.00
D4003-2-24DNA Wash Buffer (Concentrate)24 ml$33.00
D4004-1-LDNA Binding Buffer100 ml$57.00
D4003-2-48DNA Wash Buffer (Concentrate)48 ml$60.00
C1013-10Zymo-Spin VI Columns10 Pack$44.00
C1013-20Zymo-Spin VI Columns20 Pack$68.00
ZYMO RESEARCH的DNA / RNA屏蔽DNA / RNA Shield是任何生物样品的DNA和RNA传输和存储介质。DNA / RNA Shield可在环境温度下(无需冷藏或冷冻)保留样品的遗传完整性和表达特征,并完全灭活传染性因子(病毒,细菌,真菌和寄生虫)。可以直接从存储在该DNA和RNA传输和存储介质中的样品中分离核酸,而无需沉淀或去除试剂(与大多数DNA和RNA纯化试剂盒兼容)。