Cat-Nr.	100-110S
Size	5 µg
Price	75 €
Source	E. coli
Label	TAT-Fusionprotein
Formulation	lyophilized
Purity Confirmation	> 95% by SDS-PAGE & HPLC analyses
Length [aa]	304
Molecular Weight	36.2 kDa
Endotoxin Levels	< 0.1 ng/µg of protein (<1EU/µg)
Biological Activity	Testing in progress.
Species Reactivity	Human
Synonyms	Nanog-TAT
Description	Nanog is a regulatory protein that is associated with undifferentiated pluripotent cells. The expression of nanog, which is suppressed in all adult tissues, is restricted to embryonic stem cells and to certain pluripotent cancer cells. Decreased expression of nanog is strongly correlated with cell differentiation. Nanog, most likely, acts as an intracellular regulator, that helps maintain pluripotency and self renewal via a STAT3-independent pathway. The introduction of nanog, along with Sox2, Oct4, and Lin28, into primary human fibroblasts was sufficient to confer a pluripotent state upon the fibroblast genome. The reprogrammed cells thus obtained resemble ESC in morphology and gene expression. Protein transduction using TAT fusion proteins represents an alternative methodology for introducing transcription factors into primary, as well as transformed, cells. Recombinant Human Nanog-TAT is a 36.1 kDa protein, which is synthesized as a 304 amino acid polypeptide plus a 13-residue C-terminal TAT peptide.
Protein Sequence	SVDPACPQS LPCFEASDCK ESSPMPVICG PEENYPSLQM SSAEMPHTET VSPLPSSMDL LIQDSPDSST SPKGKQPTSA ENSVAKKEDK VPVKKQKTRT VFSSTQLCVL NDRFQRQKYL SLQQMQELSN ILNLSYKQVK TWFQNQRMKS KRWQKNNWPK NSNGVTQKAS APTYPSLYSS YHQGCLVNPT GNLPMWSNQT WNNSTWSNQT QNIQSWSNHS WNTQTWCTQS WNNQAWNSPF YNCGEESLQS CMQFQPNSPA SDLEAALEAA GEGLNVIQQT TRYFSTPQTM DLFLNYSMNM QPEDVGGYGR KKRRQRRR
Uniprot ID	Q9H9S0
Protein RefSeq	NP_079141.2
mRNA RefSeq	NM_024865

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