productdatasheet

Anti-CD74MonoclonalAntibody

ORDERINGINFORMATIONCatalogNo.:56451(clonePIN1)Size:100uginPBS,pH7.4,purifiedbyProteinGaffinitychromatography.

BACKGROUND

CD74isanon-polymorphictypeIIintegralmembraneprotein.IthasashortN-terminalcytoplasmictailof28aminoacidsfollowedbyasingle24-aatransmembraneregionandanapproximately150-aalumenaldomain.CD74functionsmainlyasanMHCclassIIchaperonewhichpromotesERexitofMHCclassIImoleculesbydirectingthemtoendocyticcompartments,preventingpeptidebindingintheER,andcontributingtopeptide“editing”intheMHCclassIIcompartment.CD74hasrecentlybeenshowntohavearoleasanaccessorysignalingmoleculebecauseofitshigh-affinitybindingtothepro-inflammatorycytokinemacrophagemigrationinhibitoryfactor(MIF).

SPECIFICATIONSUMMARYAntigen:SyntheticpeptidecorrespondingtothehumanCD74invariantchain(aa12-

28)conjugatedtoKLH.HostSpecies:MouseAntibodyClass:IgG1

Preservatives:0.09%sodiumazideOtheradditives:50%glycerolSPECIFICITY

ThisantibodyrecognizeshumanCD74.

APPLICATIONS

Immunoblotting:useat1ug/ml.Adoubletbandof~33-35kDaisdetected.

Immunoprecipitation:useat12ug/ml.Immunocytochemistry:workingconcentrationissampledependent.Flowcytometry:workingconcentrationissampledependent.

Thesearerecommendedconcentrations.Usershoulddetermineoptimalconcentrationsfortheirapplication.

Positivecontrol:PALAcelllysate.

DILUTIONINSTRUCTIONS

DiluteinPBSormediumwhichisidenticaltothatusedintheassaysystem.

STORAGEANDSTABILITY

Thisantibodyisstableforatleastone(1)yearat-20oC.Avoidrepeatedfreezingandthawing.

Forinvitroinvestigationaluseonly.Notforuseintherapeuticordiagnosticprocedures.

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SanDiego,CA92127

TollFree800.929.2114

Phone858.675.2405

Fax858.592.1509

info@qedbio.com

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