ORDERINGINFORMATION
CatalogNo.:43005
Format:100ulat1.0mg/mlinPBS(withoutMg2+andCa2+),pH7.4,150mMNaCl,0.02%sodiumazideand50%glycerol.Affinity-purifiedonphosphopeptide;non-phosphopeptide-reactiveantibodieswereremovedbychromatographyonnon-phosphorylatedpeptide.
BACKGROUND
PDK1phosphorylatesandactivatesPKB/AKT,PKA,PKC-zeta,RPS6KA1,andRPS6KB1andplaysaroleingeneralsignalingprocessesandindevelopment.
SPECIFICATIONSUMMARY
Antigen:Peptidesequencethatincludesphosphorylationsiteofserine241(A-N-S(p)-F-V)derivedfromhumanPDK1andconjugatedtoKLH.
Accessionno.:O15530,NP_002604.1
HostSpecies:Rabbit
Specificity:Thisantibodydetectshuman,mouse,andratendogenousPDK1onlywhenphosphorylatedatserine241.
APPLICATIONS
Westernblotting:useatdilutionof1:500-1:1,000.Abandof~63kDaisdetected.
Immunohistochemistry:useatdilutionof1:50-1:100.
DetectionofPDK1(Phospho-Ser241)in293
andPC12cellextractsuntreatedortreatedwithEGF.
Thesearerecommendedworkingdilutions.Endusershoulddetermineoptimaldilutionsfortheirapplications.
DetectionofPDK1(phospho-Ser241)inparaffin-embeddedhumanbreastcarcinomatissue.
DILUTIONINSTRUCTIONS
DiluteinPBSormediumthatisidenticaltothatusedintheassaysystem.
STORAGEANDSTABILITY
Thisantibodyisstableforatleastone(1)yearat-20oC.Canbestoredat4oCforshort-term.
Forinvitroinvestigationaluseonly.Notintendedfortherapeuticordiagnosticapplications.
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