ORDERINGINFORMATION
CatalogNo.:43003
Format:100ulat1.0mg/mlinPBS(withoutMg2+andCa2+),pH7.4,150mMNaCl,0.02%sodiumazideand50%glycerol.Affinity-purifiedonphosphopeptide;non-phosphopeptide-reactiveantibodieswereremovedbychromatographyonnon-phosphorylatedpeptide.
BACKGROUND
c-Junincombinationwithc-FosformstheAP-1earlyresponsetranscriptionfactor.ItwasfirstidentifiedastheFos-bindingproteinp39andonlylaterrediscoveredastheproductofthec-Jungene.ItisactivatedthroughdoublephosphorylationbytheJNKpathwaybuthasalsoaphosphorylation-independentfunction
SPECIFICATIONSUMMARY
Antigen:Peptidesequencethatincludesphosphorylationsiteofserine73(L-A-S(p)-P-E)derivedfromhumanc-JunandconjugatedtoKLH.
Accessionno.:P05412,NP_002219.1
HostSpecies:Rabbit
Specificity:Thisantibodydetectsendogenoushuman,mouse,andratc-Junonlywhenphosphorylatedatserine73.
APPLICATIONS
Westernblotting:useatdilutionof1:500-1:1,000.Abandof~43kDaisdetected.
Immunohistochemistry:useatdilutionof1:50-1:100.
Detectionofc-Jun(phospho-Ser73)
inPC14cellextractsuntreatedortreatedwithEGF.
Thesearerecommendedworkingdilutions.Endusershoulddetermineoptimaldilutionsfortheirapplications.
Detectionofc-Jun(phospho-Ser73)inparaffin-embeddedhumanbreastcarcinomatissue.
DILUTIONINSTRUCTIONS
DiluteinPBSormediumthatisidenticaltothatusedintheassaysystem.
STORAGEANDSTABILITY
Thisantibodyisstableforatleastone(1)yearat-20oC.Canbestoredat4oCforshort-term.
Forinvitroinvestigationaluseonly.Notintendedfortherapeuticordiagnosticapplications.
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