ORDERINGINFORMATION
CatalogNo.Size
188121mg
18812-01100ug
Format:1mg/mlinPhosphate-BufferedSaline(PBS),pH7.2,0.09%sodiumazide.
BACKGROUND
Apolyhistidine-tagconsistsofatleastsixhistidine(His)residues,oftenattheN-orC-terminusofaprotein.Polyhistidine-tagsareoftenusedforaffinitypurificationofpolyhistidine-taggedrecombinantproteinsexpressedinEscherichiacoliandotherprokaryoticexpressionsystems.
SPECIFICATIONSUMMARY
Antigen:6-His(HHHHHH)conjugatedtoKLH.
HostSpecies:Goat
Specificity:Antibodywasaffinity-purifiedonthepeptide.ThisantibodydoesnotrecognizeallN-terminal6-Histaggedproteins.
APPLICATIONS
Immunoblotting:useatadilutionof1:1,000-1:5,000.
Detectionof6-Histaggedproteinin200,100,and50ngofE.colilysatecontainingtaggedfusionproteinwith#18812diluted1:5,000.
ELISA:fordetection,useatadilutionof1:1,000-1:30,000;forcoating,useatadilutionof1:100-1:500.
Immunocytochemistry:useatadilutionof1:100-1:400.
Immunoprecipitation:useat1-4ug/mglysate.
Thesearerecommendeddilutions.Endusersshoulddetermineoptimaldilutionsfortheirapplications.
DILUTIONINSTRUCTIONS
DiluteinPBSormediumthatisidenticaltothatusedintheassaysystem.
STORAGEANDSTABILITY
Thisantibodyisstableforatleastone(1)yearat2-8oC.
Forinvitroinvestigationaluseonly.Notforuseintherapeuticordiagnosticprocedures.
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