BacterialTransglutaminaseMonoclonalAntibody

ORDERINGINFORMATIONCatalogNo.:90003(clone3C7)Format:100ug,ProteinA-purified,lyophilized,0.1MTris,0.1Mglycine,2%sucrose.

BACKGROUND

Transglutaminases(Tgases)aremultifunctionalenzymesthatarewidelydistributedamongmammals,inverte-bratesandplants.StreptomycesmobaraensisTgaseisoneofthemostusefulTgasesduetoitsratherbroadsubstratespecificityandindependenceofCa2+.TheroleofTGaseinthelife-cycleofStreptomycetesisnotfullyunderstood,butitislikelythatitfunctionsinproteincross-linkingthatfortifiestheaerialcellwallandthesporeenvelope.TheproteinstructuresoftheknownbacterialTgasesarequitedifferentfrommammalianTgasesinthattheylacksequencehomologyandhavesmallermolecularmasses.Tgasefrom

S.mobaraensisissecretedasa42.5-kDaprecursorproteinwhichisthenprocessedbytheremovalofa

45amino-acidN-terminalpeptide.

SPECIFICITY

ThisantibodyrecognizesStreptomycestransglutaminase.Itdoesnotcross-reactwithmammaliantissuetransglutaminase(Tgase2).

APPLICATIONS

Immunoblotting:useat1-10ug/ml.Abandof~38kDaisdetected.

ELISA:useat1-5ug/mlwithStreptomycesTgaseonthesolidphase.Thesearerecommendedconcentrations.Endusershoulddetermineoptimalconcentrationsfortheirapplications.

RECONSTITUTION

Reconstituteindistilledwater.

STORAGEANDSTABILITY

Thisproductisstableforatleastone(1)

oo

SPECIFICATIONSUMMARY

Antigen:RecombinantStreptomycesmobaraensistransglutaminase.

Hostspecies:Mouse.AntibodyClass:IgG1.Preservative:None.

yearat-20Cto-70C.Reconstituted

productshouldbestoredinappropriatealiquotstoavoidrepeatedfreeze-thawcycles.

Forinvitroinvestigationaluseonly.Notforuseintherapeuticordiagnosticprocedures.

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