Heme-Oxygenase1(HO-1)MonoclonalAntibody

ORDERINGINFORMATION

Catalogno.:13061(clone1F12.A6)Format:100uginPBS(1mg/ml),pH7.4.PurifiedbyProteinGaffinitychromatography.

BACKGROUND

Heme-oxygenaseisanenzymethatcatalyzeshemecatabolismtoyieldbiliverdin,iron,andcarbonmonoxide(CO).Therearethreeisoformsofheme-oxygenase:HO-1,HO-2,andHO-3.HO-1andHO-2havebeenidentifiedasthetwomajorisoformsinmammals.HO-1,alsoknownasheatshockprotein32(Hsp32)isinducedbymostoxidativestressinducers,cytokines,inflammatoryagents,andheatshock.HO-1deficiencyappearstocausereducedstressdefense,apro-inflammatorytendency,susceptibilitytoatheroscleroticlesionformation,endothelialcellinjury,andgrowthretardation.Therefore,up-regulationofHO-1isoneofthemajordefensemechanismsagainstoxidativestress.

SPECIFICATIONSUMMARY

Antigen:Syntheticpeptidecorrespondingtoaa1-30ofhumanHO-1.Accessionno.:P09601,NP_002124.1HostSpecies:MouseAntibodyClass:IgG1Specificity:Thisantibodyrecognizeshuman,mouse,andbovineHO-1.Itdoesnotcross-reactwithHO-2.

APPLICATIONS

ELISA:useat1ug/mlwithHO-1onthesolidphase.

Immunoblotting:useat0.5-1ug/ml.Abandof~32kDaisdetected.

Immunofluorescence:useat5-10ug/ml

DetectionofHO-1informaldehyde-fixedHeLacells.

Positivecontrol:HumancelllinelysatessuchasA431,A549,HeLa,HepG2,Jurkat,orMCF7.

Thesearerecommendedconcentrations.Endusershoulddetermineoptimalconcentrationsfortheirapplications.

DILUTIONINSTRUCTIONS

DiluteinPBSormediumthatisidenticaltothatusedintheassaysystem.

Heme-Oxygenase1(HO-1)MonoclonalAntibody

STORAGEANDSTABILITY

Thisantibodyisstableforatleastone(1)yearat-20oC.Avoidrepeatedfreeze-thawcycles.

Forinvitroinvestigationaluseonly.Notintendedfordiagnosticortherapeuticapplications.

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