ORDERINGINFORMATION
Catalognos.:13006-50(50ug)13006-200(200ug)
Format:ProteinA-purifiedantibodyinPBS,pH7.2,50%glycerol,0.09%sodiumazide,.
BACKGROUND
Cytochromep450isahighlyconservedanddiversefamilyofheme-containingproteinsthatfunctioninthemetabolismofnumerousendogenousandexogenoussubstrates.Thehumangenomeencodes57p450genesdividedamong18familieswhichtogetheraccountforthediversityofsubstratesmetabolized.p450istheprimaryconjugatingenzymeinphaseImetabolismanddetoxificationofendogenousandexogenouscompoundsandareprimarilylocalizedtotheinnermembranesofmitochondriaandtheER.Eukaryoticmicrosomalcytochromep450enzymesreceiveelectronsfromtheFAD-andFMN-containingenzymeNADPH:cytochromep450reductase.
SPECIFICATIONSUMMARY
Antigen:NativeratcytochromeP450.
Accessionno.:P05182
GeneID:25086
HostSpecies:Rabbit
AntibodyClass:Polyclonal
Specificity:Thisantibodyrecognizeshuman,mouse,rat,rabbit,canine,Guineapig,horseandmonkeycytochromeP450.
APPLICATIONS
Immunoblotting:Useat1-5ug/ml.Abandof~50-55kDaisdetected.
DetectionofcytochromeP450inhumanlivermicrosomes(Lane2),ratlivermicrosomes(Lane3),mouselivermicrosomes(Lane4),rabbitlivermicrosomes(Lane5).
Thesearerecommendedconcentrations.Endusersshoulddetermineoptimalconcentrationsfortheirapplication.
DILUTIONINSTRUCTIONS
DiluteinPBSormediumthatisidenticaltothatusedintheassaysystem.
STORAGEANDSTABILITY
Thisantibodyisstableforatleastone(1)yearat-20oC.Storeinappropriatealiquotstoavoidrepeatedfreeze/thawcycles.
Forinvitroinvestigationaluseonly.Notintendedfortherapeuticordiagnosticprocedures.
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