ORDERINGINFORMATION
CatalogNo.:12531(cloneN1664A)Format:100uginPBS,pH7.4,50%glycerol,0.09%sodiumazide.PurifiedbyProteinGaffinitychromatography.BACKGROUND
FreerADIcalsarehighlyreactiveandgenerallyshort-livedspecies.Hence,moststudiesoffreeradicalshavebeenrestrictedtoanalysisofendproductsformedfollowingtheirinteractionwithcellularbiomolecules.Onetechnologythathaspermitteddirectstudyoffreeradicalsiselectronspinresonance(ESR).Forveryshortlivedradicals,spintrapshavebeenemployedtogeneratelongerlivedradicalsthataremoreamenabletoESRanalysis.Themostwidelyusedofthesespintrapsis5,5-dimethyl-1-pyrroline-N-oxide(DMPO).ThereactionofDMPOwithaproteinradicalgivesrisetoanitroxideradicalwhichcanbedisproportionatetohydrozylamineandnitroneadducts.Ofthesespecies,onlythenitroneadductcanbeconsideredstable.
SPECIFICATIONSUMMARY
Antigen:5,5-dimethyl-2-(8-octanoicacid)-1-pyrrolone-N-oxideconjugatedtoovalbumin.HostSpecies:MouseAntibodyClass:IgG1Specificity:ThisantibodyrecognizesDMPO,DMPO-octanoicacid,DMPO-proteinadducts,andDMPO-DNAadducts.Itdoesnotcross-reactwithnon-adductedproteinsorDNA.Speciesindependent.APPLICATIONS
Immunoblotting:useat1-10ug/ml.Immunofluorescence:useat10ug/ml.
Thesearerecommendedconcentrations;endusershoulddetermineoptimalconcentrationsfortheirapplications.
DetectionofDMPOinmousemacrophages.
ELISA:useat1-10ug/ml
Positivecontrol:DMPOnitroneadductsofmetmyoglobin.
DILUTIONINSTRUCTIONS
DiluteinPBSormediumthatisidenticaltothatusedintheassaysystem.
STORAGEANDSTABILITY
Thisantibodyisstableforatleastone(1)yearat-20oC.Avoidmultiplefreeze-thawcycles.
Forinvitroinvestigationaluseonly.Notintendedfordiagnosticortherapeuticapplications.
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