Cataloguenumber
ENZ-277
Description
Urease(EC3.5.1.5)isanenzymethatcatalyzesthehydrolysisofureaintocarbondioxideandammonia.Thereactionoccursasfollows:(NH2)2CO+H2O=CO2+2NH3.In1926JamesSumnershowedthatureaseisaprotein.Ureaseisfoundinbacteria,yeastandseveralhigherplants.Characteristics:Activesitemetal:nickel(II);Molecularweight:480kDaor545kDaforJackBeanUrease(calculatedmassfromtheaminoacidsequence);OptimumpH:7.4;OptimumTemperature:60degreesCelsius;Enzymaticspecificity:urea;Inhibitors:heavymetals.Themulti-subunitenzymeusuallyhasa3:3(alpha:beta)stoichiometrywitha2-foldsymmetricstructure(notethattheimageabovegivesthestructureoftheasymmetricunit,onethirdofthetrueBIOLOGicalassembly).AnexceptionalureaseisfoundinHelicobacterpylori,whichcombinesfouroftheregularsixsubunitenzymesinanoveralltetrahedralassemblyof24subunits(a12b12).Thissupra-molecularassemblyisthoughttoconferadditionalstABIlityfortheenzymeinthisorganism,whichfunctionstoproduceammoniainordertoneutralisegastricacid.ThepresenceofureaseisusedinthediagnosisofHelicobacterspecies.
Source
EscherichiaColi.
PhysicalAppearance
SterileLyophilizedPowder.
Formulation
Eachmgofproteincontains420µgPotassiumPhosphateand30µgEDTANa2.
Solubility
ItisrecommendedtoreconstitutethelyophilizedUreaseinsterile18MΩ-cmH2O.
Stability
Ureasealthoughstableat4°Cfor3weeks,shouldbestoreddesiccatedbelow-18°C.
Pleasepreventfreeze-thawcycles.
Purity
Greaterthan95.0%asdeterminedby:
(a)AnalysisbyRP-HPLC.
(b)AnalysisbySDS-PAGE.
BiologicalActivity
Theactivitywasfoundtobe141U/mgpowder.
UnitDefinition
OneUnitoxidizesonemicromoleofNADHperminuteat25°C,atpH7.6.
Usage
Prospec"sproductsarefurnishedforLABORATORYRESEARCHUSEONLY.Theproductmaynotbeusedasdrugs,agriculturalorpesticidalproducts,foodadditivesorhouseholdchemicals.
ProSpec是全球著名细胞因子蛋白及相关抗体生产和研发品牌，专注于蛋白生产研发，独有细菌和哺乳动物表达和蛋白折叠技术。目前可提供细胞因子、生长因子、激素、信号蛋白、病毒抗原等近800种重组蛋白和100多种抗体。
ProSpec is an evolving biotech company providing highly purified proteins worldwide which serve the research community with cytokine-related products for cancer, apoptosis, development, endocrinology, immunology, neuroscience, proteases, and stem cell research.
Among our proteins are Interferons, Interleukins, Bone Morphogenic Proteins, Tumor Necrosis factors, Leptins, Stem Cell factors, Prolactins, Chemokines, Antibodies, Peptides, Enzymes, Viral Antigens, various growth factors such as: Platelet Derived, Epidermal, Insulin Like, Nerve, Connective Tissue, Vascular Endothelial, Fibroblast and more.....
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